pUC-GFP-AT — PlasmidDB

Plasmid Information

Purpose

"Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

Depositor

Chris Barnes

Article

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

axe/txe toxin-antitoxin cassette

Available Since

Nov. 25, 2019

Availability

Available

Sequence Information

Length

6,475 bp

Topology

circular

Molecule Type

other DNA

View on AddGene.org

Features

CDS 2

Label	Gene	Product	Position	Strand
daGFP	—	green fluorescent protein with peak exc…	4335..5046	Fwd
DNA Sequence ATGACAGCTCTGACAGAGGGAGCGAAACTGTTCGAGAAGGAGATCCCGTACATCACAGAGTTGGAAGGAGATGTCGAAGGGATGAAATTCATCATCAAGGGAGAAGGAACGGGTGACGCAACGACCGGAACGATCAAGGCCAAATACATTTGTACCACGGGCGATTTGCCTGTCCCCTGGGCGACGCTTGTAAGCACGCTCTCGTATGGTGTCCAGTGCTTCGCGAAATATCCATCGCACATTAAGGACTTTTTCAAGTCGGCCATGCCAGAAGGTTACACACAAGAACGAACCATCTCCTTTGAGGGGGACGGAGTGTATAAGACACGCGCGATGGTAACGTACGAGCGCGGGTCCATCTACAACAGGGTAACTCTTACTGGGGAGAACTTTAAGAAGGACGGGCATATCTTGCGGAAAAACGTGGCATTTCAATGTCCGCCCTCGATTCTGTATATTCTCCCGGACACGGTGAATAATGGGATCAGAGTGGAGTTCAACCAGGCATACGATATTGAGGGTGTGACTGAAAAGCTCGTCACCAAATGCAGCCAGATGAATCGCCCCCTTGCGGGATCAGCAGCCGTCCATATCCCCCGGTATCACCATATTACCTACCACACAAAACTCTCAAAAGACAGAGATGAGAGAAGGGACCATATGTGCCTGGTCGAAGTAGTGAAGGCGGTGGATCTTGATACATACCAGTAG Protein Sequence MTALTEGAKLFEKEIPYITELEGDVEGMKFIIKGEGTGDATTGTIKAKYICTTGDLPVPWATLVSTLSYGVQCFAKYPSHIKDFFKSAMPEGYTQERTISFEGDGVYKTRAMVTYERGSIYNRVTLTGENFKKDGHILRKNVAFQCPPSILYILPDTVNNGIRVEFNQAYDIEGVTEKLVTKCSQMNRPLAGSAAVHIPRYHHITYHTKLSKDRDERRDHMCLVEVVKAVDLDTYQ
NeoR/KanR	aph(3')-II (or nptII)	aminoglycoside phosphotransferase from …	5444..6239	Fwd
DNA Sequence ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCGGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGTCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCGACGACGGGCGTTCCTTGCGCGGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGTCTATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGTCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTTGTGCTTTACGGTATCGCCGCGCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA Protein Sequence MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF

misc_feature 2

Label	Gene	Product	Position	Strand
5' beta-globin insulator	—	—	57..129	Fwd
DNA Sequence CATCTTGGACCATTAGCTCCACAGGTATCTTCTTCCCTCTAGTGGTCATAACAGCAGCTTCAGCTACCTCTC
3' beta-globin insulator	—	—	2745..2817	Fwd
DNA Sequence CATTCCTTTAATGGTCTTTTCTGGACACCACTAGGGGTCAGAAGTAGTTCATCAAACTTTCTTCCCTCCCTA

polyA_signal 1

Label	Gene	Product	Position	Strand
SV40 poly(A) signal	—	—	2252..2374	Fwd
DNA Sequence TAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT

promoter 1

Label	Gene	Product	Position	Strand
recA (Delta-LexA) promoter	—	—	4250..4319	Fwd
DNA Sequence CTTGATAAGGTCCACGTAGCTGCTATAATTGCTTCAACAGAACATATTGACTATCCGGTATTACCCGGC

rep_origin 1

Label	Gene	Product	Position	Strand
ori	—	—	3042..3631	Rev
DNA Sequence TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA

terminator 5

Label	Gene	Product	Position	Strand
T7 terminator	—	—	133..180	Rev
DNA Sequence TAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
T7 terminator	—	—	2488..2535	Fwd
DNA Sequence TAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
rrnG terminator	—	—	2564..2701	Fwd
DNA Sequence GCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAA
rrnG terminator	—	—	5052..5188	Fwd
DNA Sequence CATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAA
rrnG terminator	—	—	6251..6388	Fwd
DNA Sequence GCATTGGCGCAGAAAAAAATGCCTGATGCGACGCTGCGCGTCTTATACTCCCACATATGCCAGATTCAGCAACGGATACGGCTTCCCCAACTTGCCCACTTCCATACGTGTCCTCCTTACCAGAAATTTATCCTTAA