pCS2FA CO Tol2 TPase

133032 Danio rerio Bacterial Expression

Plasmid Information

Purpose
Expression vector for production of codon optimized Tol2 transposase
Depositor
Charles Nathan Hancock
Article
Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791
Insert
Codon optimized Tol2 Transposase
Mutation
Codon Optimized
Available Since
June 15, 2020
Availability
Available

Sequence Information

Length
6,031 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 2
Label Gene Product Position Strand
Tol2 transposase transposase of the Tol2 transposon from… 1126..3076 Fwd
DNA Sequence
ATGGAAGAGGTATGTGACAGTAGTGCGGCGGCTTCTTCTACTGTTCAAAACCAACCTCAGGATCAGGAACATCCCTGGCCATATCTTCGAGAATTCTTTTCCCTGTCTGGCGTTAATAAGGATAGCTTTAAGATGAAGTGCGTGTTGTGCCTTCCGTTGAACAAGGAAATATCCGCATTCAAGTCTTCTCCTAGTAATCTCCGAAAGCACATAGAGAGGATGCACCCAAATTATCTGAAGAATTATTCAAAATTGACAGCACAGAAGAGGAAAATAGGAACTAGCACTCATGCAAGCAGTTCAAAACAACTTAAGGTCGATTCTGTGTTTCCAGTAAAGCATGTTAGTCCAGTCACAGTAAACAAAGCAATCCTTCGATACATAATTCAAGGTCTGCATCCATTCTCAACGGTGGATCTTCCCAGCTTCAAGGAACTTATTTCCACGCTGCAGCCCGGCATTTCAGTAATCACCAGGCCAACATTGCGGTCCAAGATCGCCGAGGCTGCGCTGATTATGAAACAGAAGGTTACTGCCGCGATGTCAGAAGTCGAGTGGATAGCTACTACTACCGACTGCTGGACTGCAAGGCGAAAAAGTTTCATTGGCGTGACAGCACACTGGATCAATCCTGGTAGCCTCGAGAGGCACTCCGCTGCCCTGGCTTGTAAACGCCTTATGGGGAGTCATACTTTTGAAGTCCTTGCATCTGCAATGAATGATATCCACAGTGAGTATGAGATTCGTGACAAGGTTGTTTGTACTACAACAGATTCAGGTAGCAACTTTATGAAAGCTTTCCGTGTATTCGGAGTTGAGAACAATGATATCGAAACAGAAGCCAGAAGGTGCGAGAGTGACGACACGGACTCCGAAGGGTGTGGTGAGGGTTCCGATGGCGTTGAATTTCAGGACGCGTCTAGGGTTCTTGACCAAGATGACGGCTTTGAATTTCAGTTGCCGAAACATCAGAAATGTGCGTGCCATCTGCTCAATCTTGTAAGTTCAGTCGACGCCCAGAAAGCTCTTTCTAACGAACACTATAAGAAACTCTATAGAAGCGTCTTCGGGAAGTGTCAAGCGCTCTGGAACAAGTCCAGCCGTTCTGCACTTGCGGCAGAGGCTGTAGAATCCGAAAGTCGCCTGCAACTGTTGCGACCCAATCAAACGCGATGGAACTCAACCTTCATGGCCGTGGACCGGATTCTCCAGATCTGCAAGGAAGCGGGCGAGGGTGCCCTCCGCAATATTTGTACTTCTTTGGAGGTACCAATGTTTAATCCGGCAGAAATGCTTTTCTTGACGGAATGGGCCAATACAATGCGTCCGGTGGCAAAGGTTCTTGACATTCTGCAGGCGGAAACAAATACTCAACTCGGTTGGCTGCTGCCGAGCGTACACCAACTCTCCCTGAAGCTCCAGCGTCTCCATCATAGTTTGCGGTACTGCGACCCGCTGGTAGATGCGCTCCAGCAAGGGATTCAGACTCGCTTTAAACATATGTTCGAAGATCCAGAGATAATCGCTGCGGCGATACTGCTTCCCAAATTCAGAACAAGCTGGACTAATGACGAGACCATCATCAAGCGGGGGATGGATTACATACGGGTACACCTTGAACCACTCGACCATAAGAAAGAACTGGCAAACTCTTCTTCCGATGATGAGGACTTCTTCGCTTCACTTAAACCAACGACGCATGAAGCGTCAAAGGAGCTCGACGGTTATCTCGCGTGCGTTTCAGACACCAGAGAATCCCTCTTGACCTTTCCCGCGATATGCAGCTTGTCCATAAAGACCAATACGCCACTGCCTGCTAGCGCTGCCTGTGAACGTCTCTTTTCCACTGCCGGGTTGCTCTTTAGCCCAAAGAGAGCCAGGCTTGACACGAACAACTTTGAGAACCAACTTCTCTTGAAGCTCAACCTTAGGTTTTACAACTTCGAGTAG
Protein Sequence
MEEVCDSSAAASSTVQNQPQDQEHPWPYLREFFSLSGVNKDSFKMKCVLCLPLNKEISAFKSSPSNLRKHIERMHPNYLKNYSKLTAQKRKIGTSTHASSSKQLKVDSVFPVKHVSPVTVNKAILRYIIQGLHPFSTVDLPSFKELISTLQPGISVITRPTLRSKIAEAALIMKQKVTAAMSEVEWIATTTDCWTARRKSFIGVTAHWINPGSLERHSAALACKRLMGSHTFEVLASAMNDIHSEYEIRDKVVCTTTDSGSNFMKAFRVFGVENNDIETEARRCESDDTDSEGCGEGSDGVEFQDASRVLDQDDGFEFQLPKHQKCACHLLNLVSSVDAQKALSNEHYKKLYRSVFGKCQALWNKSSRSALAAEAVESESRLQLLRPNQTRWNSTFMAVDRILQICKEAGEGALRNICTSLEVPMFNPAEMLFLTEWANTMRPVAKVLDILQAETNTQLGWLLPSVHQLSLKLQRLHHSLRYCDPLVDALQQGIQTRFKHMFEDPEIIAAAILLPKFRTSWTNDETIIKRGMDYIRVHLEPLDHKKELANSSSDDEDFFASLKPTTHEASKELDGYLACVSDTRESLLTFPAICSLSIKTNTPLPASAACERLFSTAGLLFSPKRARLDTNNFENQLLLKLNLRFYNFE
AmpR bla beta-lactamase 4560..5421 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
polyA_signal 1
Label Gene Product Position Strand
SV40 poly(A) signal 3113..3248 Fwd
DNA Sequence
GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
promoter 5
Label Gene Product Position Strand
CMV IE94 promoter 46..1031 Fwd
DNA Sequence
GTCGACCGCCAATTCAATATGGCGTATATGGACTCATGCCAATTCAATATGGTGGATCTGGACCTGTGCCAATTCAATATGGCGTATATGGACTCGTGCCAATTCAATATGGTGGATCTGGACCCCAGCCAATTCAATATGGCGGACTTGGCACCATGCCAATTCAATATGGCGGACCTGGCACTGTGCCAACTGGGGAGGGGTCTACTTGGCACGGTGCCAAGTTTGAGGAGGGGTCTTGGCCCTGTGCCAAGTCCGCCATATTGAATTGGCATGGTGCCAATAATGGCGGCCATATTGGCTATATGCCAGGATCAATATATAGGCAATATCCAATATGGCCCTATGCCAATATGGCTATTGGCCAGGTTCAATACTATGTATTGGCCCTATGCCATATAGTATTCCATATATGGGTTTTCCTATTGACGTAGATAGCCCCTCCCAATGGGCGGTCCCATATACCATATATGGGGCTTCCTAATACCGCCCATAGCCACTCCCCCATTGACGTCAATGGTCTCTATATATGGTCTTTCCTATTGACGTCATATGGGCGGTCCTATTGACGTATATGGCGCCTCCCCCATTGACGTCAATTACGGTAAATGGCCCGCCTGGCTCAATGCCCATTGACGTCAATAGGACCACCCACCATTGACGTCAATGGGATGGCTCATTGCCCATTCATATCCGTTCTCACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCACTTGGCAGTACATCAATATCTATTAATAGTAACTTGGCAAGTACATTACTATTGGAAGTACGCCAGGGTACATTGGCAGTACTCCCATTGACGTCAATGGCGGTAAATGGCCCGCGATGGCTGCCAAGTACATCCCCATTGACGTCAATGGGGAGGGGCAATGACGCAAATGGGCGTTCCATTGACGTAAATGGGCGGTAGGCGTGCCTAATGGGAGGTCTATATAAGCAATGCTCGTTTAGGGAAC
SP6 promoter 1065..1084 Fwd
DNA Sequence
ATTTAGGTGACACTATAGA
T3 promoter 3360..3379 Rev
DNA Sequence
AATTAACCCTCACTAAAGG
lac promoter 3447..3478 Rev
DNA Sequence
TTTACACTTTATGCTCCCGGCTCGTATGTTG
AmpR promoter bla 5421..5526 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 2
Label Gene Product Position Strand
lac operator 3423..3440 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 3492..3514 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 2
Label Gene Product Position Strand
ori 3801..4390 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
f1 ori 5551..6007 Rev
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTT