pTone-gata2aECE-Kaede

132976 Danio rerio Bacterial Expression

Plasmid Information

Purpose
gata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backbone
Depositor
Nathan Lawson
Article
Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d
Available Since
Dec. 4, 2019
Availability
Available

Sequence Information

Length
4,407 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
Kaede — Kaede green-to-red photoconvertible flu… 715..1393 Fwd
DNA Sequence
ATGAGTCTGATTAAACCAGAAATGAAGATCAAGCTGCTTATGGAAGGCAATGTAAACGGGCACCAGTTTGTTATTGAGGGAGATGGAAAAGGCCATCCTTTTGAGGGAAAACAGAGTATGGACCTTGTAGTCAAAGAAGGCGCACCTCTCCCTTTTGCCTACGATATCTTGACAACAGCATTCCATTATGGTAACAGGGTTTTTGCTAAATACCCAGACCATATACCAGACTACTTCAAGCAGTCGTTTCCCAAAGGGTTTTCTTGGGAGCGAAGCCTGATGTTCGAGGACGGGGGCGTTTGCATCGCTACAAATGACATAACACTGAAAGGAGACACTTTTTTTAACAAAGTTCGATTTGATGGCGTAAACTTTCCCCCAAATGGTCCTGTTATGCAGAAGAAGACTCTGAAATGGGAGGCATCCACTGAGAAAATGTATTTGCGTGATGGAGTGTTGACGGGCGATATTACCATGGCTCTGCTGCTTAAAGGAGATGTCCATTACCGATGTGACTTCAGAACTACTTACAAATCTAGGCAGGAGGGTGTCAAGTTGCCAGGATATCACTTTGTCGATCACTGCATCAGCATATTGAGGCATGACAAAGACTACAACGAGGTTAAGCTGTATGAGCATGCTGTTGCCCATTCTGGATTGCCGGACAACGTCAAGTAA
Protein Sequence
MSLIKPEMKIKLLMEGNVNGHQFVIEGDGKGHPFEGKQSMDLVVKEGAPLPFAYDILTTAFHYGNRVFAKYPDHIPDYFKQSFPKGFSWERSLMFEDGGVCIATNDITLKGDTFFNKVRFDGVNFPPNGPVMQKKTLKWEASTEKMYLRDGVLTGDITMALLLKGDVHYRCDFRTTYKSRQEGVKLPGYHFVDHCISILRHDKDYNEVKLYEHAVAHSGLPDNVK
AmpR bla beta-lactamase 3033..3894 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
polyA_signal 1
Label Gene Product Position Strand
SV40 poly(A) signal — — 1485..1620 Fwd
DNA Sequence
GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
promoter 2
Label Gene Product Position Strand
lac promoter — — 1920..1951 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
AmpR promoter bla — 3894..3999 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 6
Label Gene Product Position Strand
attB4 mutant version of attB — 314..335 Fwd
DNA Sequence
CAACTTTGTATAGAAAAGTTG
attB1 mutant version of attB — 673..698 Fwd
DNA Sequence
GCAAGTTTGTACAAAAAAGCAGGCT
attB2 mutant version of attB — 1393..1418 Rev
DNA Sequence
CCCACTTTGTACAAGAAAGCTGGGT
attB3 mutant version of attB — 1443..1464 Rev
DNA Sequence
CAACTATGTATAATAAAGTTG
lac operator — — 1896..1913 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site — — 1965..1987 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 1
Label Gene Product Position Strand
ori — — 2274..2863 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA