pTone-gata2aECE-nsGFP

132975 Danio rerio Bacterial Expression

Plasmid Information

Purpose
gata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backbone
Depositor
Nathan Lawson
Article
Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d
Tags
6x myc and SV40 NLS
Available Since
Dec. 4, 2019
Availability
Available

Sequence Information

Length
4,789 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 9
Label Gene Product Position Strand
SV40 NLS nuclear localization signal of SV40 (si… 450..471 Fwd
DNA Sequence
CCTAAGAAGAAGAGGAAGGTG
Protein Sequence
PKKKRKV
Myc Myc (human c-Myc proto-oncogene) epitop… 498..528 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc Myc (human c-Myc proto-oncogene) epitop… 537..567 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc Myc (human c-Myc proto-oncogene) epitop… 576..606 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc Myc (human c-Myc proto-oncogene) epitop… 615..645 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc Myc (human c-Myc proto-oncogene) epitop… 654..684 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
Myc Myc (human c-Myc proto-oncogene) epitop… 717..747 Fwd
DNA Sequence
GAGCAAAAGCTCATTTCTGAAGAGGACTTG
Protein Sequence
EQKLISEEDL
GFP11 11th beta-strand of superfolder GFP 1410..1458 Fwd
DNA Sequence
CGCGACCACATGGTGCTGCATGAGTATGTCAACGCTGCGGGAATTACC
Protein Sequence
RDHMVLHEYVNAAGIT
AmpR bla beta-lactamase 3153..4014 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
polyA_signal 1
Label Gene Product Position Strand
SV40 poly(A) signal 1605..1740 Fwd
DNA Sequence
GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
promoter 2
Label Gene Product Position Strand
lac promoter 2040..2071 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
AmpR promoter bla 4014..4119 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 6
Label Gene Product Position Strand
attB4 mutant version of attB 52..73 Fwd
DNA Sequence
CAACTTTGTATAGAAAAGTTG
attB1 mutant version of attB 411..436 Fwd
DNA Sequence
GCAAGTTTGTACAAAAAAGCAGGCT
attB2 mutant version of attB 1513..1538 Rev
DNA Sequence
CCCACTTTGTACAAGAAAGCTGGGT
attB3 mutant version of attB 1563..1584 Rev
DNA Sequence
CAACTATGTATAATAAAGTTG
lac operator 2016..2033 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 2085..2107 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 1
Label Gene Product Position Strand
ori 2394..2983 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA