pET-His6-FnCas9GFP

130966 Synthetic Bacterial Expression

Plasmid Information

Purpose
expression of FnCas9-GFP in bacterial cells
Depositor
Debojyoti Chakraborty
Article
Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11
Insert
HA-NLS-FnCas9
Tags
6xHis-mEGFP-TEV-HA-NLS
Promoter
T7
Available Since
Nov. 7, 2019
Availability
Available

Sequence Information

Length
11,029 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 10
Label Gene Product Position Strand
KanR aph(3')-Ia aminoglycoside phosphotransferase 548..1364 Rev
DNA Sequence
ATGAGCCATATTCAACGGGAAACGTCTTGCTCTAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAACATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
rop rop Rop protein, which maintains plasmids a… 2500..2692 Rev
DNA Sequence
GTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGA
Protein Sequence
MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL
lacI lacI lac repressor 3500..4583 Rev
DNA Sequence
GTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGTATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA
Protein Sequence
MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
6xHis 6xHis affinity tag 5068..5086 Fwd
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH
SiriusGFP enhanced GFP (EGFP) derivative that exh… 5095..5809 Fwd
DNA Sequence
GTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGCGCGGCGAGGGCGAGGGCGATGCCACCAACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTCCTTCAAGGACGACGGCACCTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTTCAACAGCCACAACGTCTATATCACGGCCGACAAGCAGAAGAACGGCATCAAGGCGAACTTCAAGATCCGCCACAACGTCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAG
Protein Sequence
VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
TEV site tobacco etch virus (TEV) protease recog… 5818..5839 Fwd
DNA Sequence
GAAAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
HA HA (human influenza hemagglutinin) epit… 5848..5875 Fwd
DNA Sequence
TACCCATACGATGTTCCAGATTACGCT
Protein Sequence
YPYDVPDYA
SV40 NLS nuclear localization signal of SV40 (si… 5878..5899 Fwd
DNA Sequence
CCGAAGAAAAAGCGCAAGGTC
Protein Sequence
PKKKRKV
FnCas9 Cas9 endonuclease from the Francisella … 5908..10792 Fwd
DNA Sequence
AATTTTAAGATCCTGCCTATCGCAATCGACCTGGGCGTCAAGAATACTGGCGTGTTTAGTGCTTTTTATCAGAAGGGGACCTCACTGGAGAGACTGGACAATAAGAACGGAAAAGTGTATGAACTGTCCAAGGATTCTTACACTCTGCTGATGAACAATAGGACCGCACGGAGACACCAGAGGCGAGGAATTGACAGGAAACAGCTGGTGAAGCGCCTGTTCAAACTGATCTGGACAGAGCAGCTGAACCTGGAATGGGATAAGGACACTCAGCAGGCCATCAGCTTCCTGTTTAATCGACGGGGATTCTCTTTTATTACTGACGGCTATAGTCCTGAGTACCTGAACATCGTGCCAGAACAGGTCAAGGCAATCCTGATGGACATTTTCGACGATTATAATGGCGAGGACGATCTGGATTCCTACCTGAAACTGGCCACAGAGCAAGAGAGTAAGATCAGCGAAATCTACAACAAGCTGATGCAGAAGATCCTGGAGTTCAAGCTGATGAAACTGTGCACCGACATCAAGGACGATAAAGTGAGTACCAAGACACTGAAAGAGATCACAAGCTACGAGTTCGAACTGCTGGCCGATTATCTGGCTAACTACAGCGAATCCCTGAAGACCCAGAAATTTTCCTACACAGACAAGCAGGGCAATCTGAAAGAGCTGTCTTACTACCACCATGATAAGTACAACATCCAGGAGTTCCTGAAGAGACACGCCACCATCAATGACAGGATTCTGGATACACTGCTGACTGACGATCTGGACATCTGGAACTTCAACTTCGAGAAGTTCGATTTCGACAAGAACGAGGAAAAACTGCAGAATCAGGAAGATAAGGACCACATTCAGGCTCATCTGCACCATTTCGTGTTTGCAGTCAATAAGATCAAAAGCGAGATGGCATCCGGCGGGCGCCATCGAAGCCAGTACTTCCAGGAAATCACCAACGTGCTGGACGAGAACAATCACCAGGAAGGCTACCTGAAAAACTTCTGTGAGAATCTGCATAACAAGAAGTACAGCAATCTGTCCGTGAAGAATCTGGTCAACCTGATTGGAAATCTGTCCAACCTGGAACTGAAGCCCCTGCGCAAATACTTCAACGACAAGATCCACGCTAAAGCAGACCATTGGGATGAGCAGAAGTTTACTGAAACCTATTGCCACTGGATTCTGGGCGAGTGGCGGGTGGGGGTCAAGGATCAGGACAAGAAAGACGGCGCAAAGTATTCTTACAAGGACCTGTGTAACGAGCTGAAGCAGAAAGTGACTAAGGCCGGGCTGGTGGACTTCCTGCTGGAGCTGGACCCCTGCCGAACCATTCCACCTTACCTGGACAACAATAACAGAAAGCCACCCAAATGTCAGAGCCTGATCCTGAATCCCAAGTTTCTGGATAATCAGTATCCTAACTGGCAGCAGTACCTGCAGGAGCTGAAGAAACTGCAGTCAATCCAGAACTACCTGGACAGCTTCGAAACCGATCTGAAGGTGCTGAAAAGCTCCAAGGACCAGCCTTACTTCGTCGAGTACAAGTCTAGTAACCAGCAGATCGCTTCCGGCCAGCGGGATTACAAGGATCTGGACGCAAGAATCCTGCAGTTCATTTTTGACAGGGTGAAGGCCTCTGATGAGCTGCTGCTGAACGAAATCTATTTCCAGGCAAAGAAACTGAAGCAGAAAGCCTCAAGCGAGCTGGAAAAGCTGGAGTCCTCTAAGAAACTGGACGAAGTGATCGCTAACTCTCAGCTGAGTCAGATTCTGAAGTCTCAGCACACAAATGGAATCTTCGAGCAGGGCACTTTTCTGCATCTGGTGTGCAAATACTATAAGCAGCGACAGAGAGCCAGGGACAGCCGCCTGTACATCATGCCTGAATATCGATACGATAAGAAACTGCACAAGTACAACAACACCGGCCGCTTTGACGATGACAACCAGCTGCTGACATATTGTAATCATAAGCCCCGGCAGAAAAGATACCAGCTGCTGAACGACCTGGCAGGAGTGCTGCAGGTCTCTCCTAATTTTCTGAAGGATAAAATCGGGTCCGATGACGATCTGTTCATTTCTAAGTGGCTGGTGGAGCACATCCGGGGCTTTAAGAAGGCCTGCGAAGACAGCCTGAAAATCCAGAAGGATAACAGGGGACTGCTGAATCATAAGATCAACATTGCACGCAATACCAAGGGCAAATGCGAGAAAGAAATCTTCAACCTGATCTGTAAGATTGAGGGGAGCGAAGACAAGAAAGGGAATTATAAGCACGGACTGGCCTACGAGCTGGGAGTGCTGCTGTTCGGAGAGCCAAACGAGGCCAGCAAGCCCGAATTTGATAGGAAAATCAAGAAATTCAATTCAATCTACAGCTTTGCCCAGATCCAGCAGATTGCCTTTGCTGAGAGGAAGGGGAATGCAAACACATGCGCCGTGTGTAGTGCAGACAACGCCCATCGCATGCAGCAGATCAAAATTACTGAGCCAGTCGAAGACAATAAGGATAAAATCATTCTGTCAGCAAAGGCACAGCGACTGCCTGCAATCCCAACCCGAATTGTGGATGGAGCTGTCAAGAAAATGGCTACAATTCTGGCAAAGAATATCGTGGACGATAATTGGCAGAACATTAAGCAGGTCCTGAGCGCAAAACACCAGCTGCATATCCCAATCATTACCGAGTCCAACGCCTTCGAGTTTGAACCCGCTCTGGCAGACGTGAAGGGCAAATCTCTGAAGGATAGAAGGAAGAAAGCCCTGGAGCGAATTAGTCCCGAAAACATCTTCAAGGATAAGAACAACAGAATCAAGGAGTTTGCTAAGGGGATTTCCGCCTACTCTGGAGCTAACCTGACAGATGGGGACTTCGATGGAGCAAAGGAGGAACTGGATCACATCATTCCTCGCAGCCATAAGAAATATGGCACTCTGAACGACGAGGCTAATCTGATTTGCGTGACCCGGGGCGATAATAAGAACAAAGGGAACCGGATCTTCTGTCTGAGAGACCTGGCCGATAATTACAAGCTGAAACAGTTTGAGACCACAGACGATCTGGAGATCGAAAAGAAAATTGCCGACACCATCTGGGATGCTAATAAGAAGGACTTCAAGTTCGGAAACTATCGGAGCTTCATCAATCTGACACCTCAGGAGCAGAAAGCATTCAGACACGCCCTGTTTCTGGCTGATGAAAACCCAATCAAGCAGGCAGTGATCAGAGCCATTAATAACCGCAACCGAACCTTCGTGAATGGCACACAGAGGTATTTTGCTGAGGTCCTGGCAAATAACATCTACCTGCGCGCCAAGAAAGAAAATCTGAACACTGACAAGATCAGCTTCGATTACTTTGGAATCCCTACCATTGGAAACGGCCGAGGGATCGCTGAGATTCGGCAGCTGTATGAAAAGGTGGACAGTGATATCCAGGCCTACGCTAAAGGCGACAAGCCACAGGCCTCTTATAGTCACCTGATTGATGCTATGCTGGCATTCTGCATCGCCGCTGACGAGCATCGGAACGATGGATCTATTGGCCTGGAAATCGACAAAAACTATAGTCTGTACCCTCTGGATAAGAATACTGGCGAGGTGTTCACCAAAGACATCTTTTCACAGATCAAGATTACCGACAACGAGTTCAGCGATAAGAAACTGGTCAGAAAGAAAGCTATTGAAGGGTTTAACACACACAGACAGATGACTAGGGATGGAATCTATGCAGAGAATTACCTGCCTATCCTGATTCATAAGGAGCTGAACGAAGTGAGGAAGGGGTACACATGGAAAAATTCCGAGGAAATCAAAATTTTCAAGGGAAAGAAATACGACATCCAGCAGCTGAATAACCTGGTGTATTGTCTGAAGTTTGTGGACAAACCAATCAGTATTGATATCCAGATTTCAACCCTGGAGGAACTGAGAAACATCCTGACTACCAATAACATTGCAGCCACTGCCGAGTACTATTACATTAATCTGAAAACCCAGAAGCTGCACGAGTATTACATCGAAAATTACAACACAGCCCTGGGGTATAAGAAATACAGCAAGGAGATGGAGTTCCTGAGGTCCCTGGCTTATAGGTCTGAGCGCGTGAAGATCAAAAGTATTGACGATGTCAAGCAGGTCCTGGACAAGGATTCAAACTTCATCATCGGAAAGATCACACTGCCCTTCAAGAAAGAGTGGCAGCGACTGTACCGGGAATGGCAGAACACAACTATCAAAGACGATTATGAGTTTCTGAAGAGCTTCTTTAATGTGAAGTCCATTACTAAACTGCACAAGAAAGTCCGGAAAGACTTCTCTCTGCCCATCAGTACAAACGAGGGCAAGTTTCTGGTGAAGAGAAAAACTTGGGATAATAACTTCATCTACCAGATTCTGAATGACTCAGATAGCAGGGCAGACGGGACTAAACCCTTCATTCCTGCCTTTGATATCAGCAAGAACGAGATTGTGGAAGCCATCATTGACAGTTTCACCTCAAAAAACATCTTTTGGCTGCCAAAGAATATTGAGCTGCAGAAGGTGGACAACAAGAACATCTTCGCCATTGATACCAGCAAGTGGTTTGAGGTCGAAACACCATCCGACCTGCGCGATATCGGCATTGCTACCATTCAGTACAAGATCGACAATAACTCACGCCCCAAGGTGCGAGTCAAACTGGATTACGTGATCGACGATGACAGCAAGATTAACTATTTCATGAATCACTCACTGCTGAAGAGCCGGTATCCCGACAAAGTCCTGGAGATCCTGAAGCAGAGCACAATCATTGAGTTCGAAAGTTCAGGGTTTAACAAAACTATTAAGGAGATGCTGGGAATGAAGCTGGCCGGCATCTACAATGAAACCTCCAATAAC
Protein Sequence
NFKILPIAIDLGVKNTGVFSAFYQKGTSLERLDNKNGKVYELSKDSYTLLMNNRTARRHQRRGIDRKQLVKRLFKLIWTEQLNLEWDKDTQQAISFLFNRRGFSFITDGYSPEYLNIVPEQVKAILMDIFDDYNGEDDLDSYLKLATEQESKISEIYNKLMQKILEFKLMKLCTDIKDDKVSTKTLKEITSYEFELLADYLANYSESLKTQKFSYTDKQGNLKELSYYHHDKYNIQEFLKRHATINDRILDTLLTDDLDIWNFNFEKFDFDKNEEKLQNQEDKDHIQAHLHHFVFAVNKIKSEMASGGRHRSQYFQEITNVLDENNHQEGYLKNFCENLHNKKYSNLSVKNLVNLIGNLSNLELKPLRKYFNDKIHAKADHWDEQKFTETYCHWILGEWRVGVKDQDKKDGAKYSYKDLCNELKQKVTKAGLVDFLLELDPCRTIPPYLDNNNRKPPKCQSLILNPKFLDNQYPNWQQYLQELKKLQSIQNYLDSFETDLKVLKSSKDQPYFVEYKSSNQQIASGQRDYKDLDARILQFIFDRVKASDELLLNEIYFQAKKLKQKASSELEKLESSKKLDEVIANSQLSQILKSQHTNGIFEQGTFLHLVCKYYKQRQRARDSRLYIMPEYRYDKKLHKYNNTGRFDDDNQLLTYCNHKPRQKRYQLLNDLAGVLQVSPNFLKDKIGSDDDLFISKWLVEHIRGFKKACEDSLKIQKDNRGLLNHKINIARNTKGKCEKEIFNLICKIEGSEDKKGNYKHGLAYELGVLLFGEPNEASKPEFDRKIKKFNSIYSFAQIQQIAFAERKGNANTCAVCSADNAHRMQQIKITEPVEDNKDKIILSAKAQRLPAIPTRIVDGAVKKMATILAKNIVDDNWQNIKQVLSAKHQLHIPIITESNAFEFEPALADVKGKSLKDRRKKALERISPENIFKDKNNRIKEFAKGISAYSGANLTDGDFDGAKEELDHIIPRSHKKYGTLNDEANLICVTRGDNKNKGNRIFCLRDLADNYKLKQFETTDDLEIEKKIADTIWDANKKDFKFGNYRSFINLTPQEQKAFRHALFLADENPIKQAVIRAINNRNRTFVNGTQRYFAEVLANNIYLRAKKENLNTDKISFDYFGIPTIGNGRGIAEIRQLYEKVDSDIQAYAKGDKPQASYSHLIDAMLAFCIAADEHRNDGSIGLEIDKNYSLYPLDKNTGEVFTKDIFSQIKITDNEFSDKKLVRKKAIEGFNTHRQMTRDGIYAENYLPILIHKELNEVRKGYTWKNSEEIKIFKGKKYDIQQLNNLVYCLKFVDKPISIDIQISTLEELRNILTTNNIAATAEYYYINLKTQKLHEYYIENYNTALGYKKYSKEMEFLRSLAYRSERVKIKSIDDVKQVLDKDSNFIIGKITLPFKKEWQRLYREWQNTTIKDDYEFLKSFFNVKSITKLHKKVRKDFSLPISTNEGKFLVKRKTWDNNFIYQILNDSDSRADGTKPFIPAFDISKNEIVEAIIDSFTSKNIFWLPKNIELQKVDNKNIFAIDTSKWFEVETPSDLRDIGIATIQYKIDNNSRPKVRVKLDYVIDDDSKINYFMNHSLLKSRYPDKVLEILKQSTIIEFESSGFNKTIKEMLGMKLAGIYNETSNN
6xHis 6xHis affinity tag 10861..10879 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
promoter 2
Label Gene Product Position Strand
lacI promoter lacI 4583..4661 Rev
DNA Sequence
GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT
T7 promoter 4969..4988 Fwd
DNA Sequence
TAATACGACTCACTATAGG
protein_bind 1
Label Gene Product Position Strand
lac operator 4988..5013 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
rep_origin 2
Label Gene Product Position Strand
f1 ori 0..456 Fwd
DNA Sequence
ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAACTAGTAACGTTTACAATTT
ori 1485..2074 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 1
Label Gene Product Position Strand
T7 terminator 10945..10993 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG