pAC1748

127982 Synthetic Bacterial Expression

Plasmid Information

Purpose
AMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)
Depositor
Uffe Mortensen
Article
Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
Insert
Cpf1
Tags
SV40 NLS
Mutation
codon optimized for Aspergillus nidulans
Promoter
Aspergillus nidulans tef1 promoter
Available Since
Dec. 11, 2019
Availability
Available

Sequence Information

Length
16,124 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 5
Label Gene Product Position Strand
AmpR bla beta-lactamase 0..16124 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGTAGCGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCGATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
LbCpf1 — CRISPR-associated protein from Lachnosp… 8022..11706 Fwd
DNA Sequence
ATGTCCAAGCTCGAGAAGTTCACCAACTGCTACTCCCTCAGCAAGACCCTCCGCTTCAAGGCTATCCCCGTCGGCAAGACCCAAGAGAATATCGATAACAAGCGCCTCCTCGTCGAGGATGAGAAGCGCGCCGAGGATTACAAGGGCGTCAAGAAGCTCCTCGATCGCTACTACCTCTCCTTCATCAACGATGTCCTCCACTCCATCAAGCTCAAGAACCTCAACAACTACATCTCCCTGTTCCGCAAGAAAACCCGCACCGAGAAAGAGAACAAAGAGCTGGAAAACCTCGAGATCAACCTCCGCAAAGAGATCGCCAAGGCCTTCAAGGGCAACGAGGGCTACAAGTCCCTGTTCAAGAAGGATATCATCGAAACCATCCTGCCTGAGTTCCTCGATGATAAGGATGAGATCGCCCTCGTCAACTCCTTCAACGGCTTCACTACCGCCTTCACCGGTTTCTTCGATAACCGCGAAAACATGTTCTCCGAGGAAGCCAAGTCCACCTCTATCGCCTTCCGCTGCATCAACGAGAACTTGACCCGCTACATCAGCAACATGGATATCTTCGAGAAGGTCGATGCCATCTTCGACAAGCACGAGGTCCAAGAGATCAAAGAAAAGATCCTCAACTCCGATTACGATGTCGAGGATTTCTTCGAGGGCGAGTTCTTCAACTTCGTGCTCACCCAAGAGGGCATCGATGTCTACAACGCCATCATCGGCGGCTTCGTCACCGAGTCCGGCGAGAAGATCAAGGGCCTCAACGAGTACATCAACCTCTACAATCAAAAGACCAAGCAGAAGCTCCCCAAGTTCAAGCCCCTCTACAAGCAGGTCCTCTCCGATCGCGAGTCCCTGTCCTTCTACGGCGAGGGTTACACCTCCGATGAGGAAGTCCTCGAGGTCTTTCGCAACACCCTCAACAAGAACTCCGAGATCTTCTCCAGCATCAAGAAGTTGGAGAAGCTTTTCAAGAACTTCGATGAGTACTCCTCCGCCGGCATCTTCGTCAAGAACGGCCCTGCTATCTCCACCATCTCCAAGGACATCTTCGGCGAGTGGAACGTCATCCGCGATAAGTGGAACGCCGAGTACGATGACATCCACCTCAAGAAGAAGGCCGTCGTTACCGAGAAGTACGAGGATGATCGCCGCAAGTCCTTCAAGAAGATCGGCTCCTTCAGCCTCGAGCAGCTCCAAGAGTACGCCGATGCCGATCTCTCCGTCGTCGAGAAGCTGAAAGAGATCATCATCCAGAAGGTGGACGAGATCTACAAGGTCTACGGCTCCTCTGAGAAGCTGTTCGATGCCGACTTCGTCCTCGAAAAGTCCCTGAAGAAGAACGACGCCGTCGTCGCTATCATGAAGGATCTGCTCGATTCCGTGAAGTCGTTCGAGAACTACATCAAGGCTTTCTTCGGTGAGGGCAAAGAGACTAACCGCGACGAGAGCTTCTACGGTGATTTCGTCCTGGCCTACGATATCCTCCTCAAGGTCGATCACATCTACGATGCTATCCGCAACTACGTCACCCAGAAGCCTTACTCCAAGGATAAGTTCAAGTTGTACTTCCAGAATCCTCAGTTCATGGGCGGCTGGGACAAAGACAAAGAAACCGATTACCGCGCCACCATCCTCCGCTACGGCAGCAAGTACTACCTCGCCATCATGGATAAGAAATACGCCAAGTGCCTCCAAAAGATCGATAAGGACGATGTCAACGGCAACTACGAAAAGATCAACTACAAGCTGCTCCCCGGTCCTAACAAGATGCTGCCCAAGGTGTTCTTCAGCAAGAAATGGATGGCCTACTACAACCCCTCCGAGGATATCCAGAAGATCTATAAGAACGGCACCTTCAAAAAGGGCGATATGTTCAACCTCAACGATTGCCACAAGCTCATCGATTTCTTCAAGGATTCCATCTCGCGCTACCCCAAGTGGTCCAACGCCTACGATTTCAACTTCTCCGAGACTGAGAAGTATAAGGATATCGCTGGCTTCTACCGTGAGGTCGAGGAACAGGGTTACAAGGTGTCCTTCGAGTCCGCCTCCAAGAAAGAGGTCGATAAGCTCGTCGAAGAGGGCAAGCTCTACATGTTCCAAATCTACAACAAGGATTTCTCCGATAAGTCCCACGGCACCCCTAACCTCCACACCATGTACTTCAAGCTGCTGTTCGACGAGAACAACCACGGCCAGATCCGCCTCTCCGGCGGTGCCGAGCTGTTCATGCGCCGTGCTAGCCTGAAGAAAGAAGAGTTGGTCGTCCATCCTGCTAACTCCCCGATCGCCAACAAGAATCCTGATAACCCCAAGAAAACTACCACCTTGTCCTACGACGTCTACAAGGACAAGCGCTTCAGCGAGGATCAGTACGAGCTGCACATCCCTATCGCCATCAACAAGTGCCCTAAGAACATCTTCAAGATCAATACCGAGGTCCGCGTCCTCCTGAAGCACGATGACAACCCCTACGTCATCGGTATCGATCGCGGCGAGCGCAACCTCCTCTACATCGTCGTCGTCGATGGCAAGGGTAACATCGTCGAGCAGTACTCCCTGAACGAAATCATCAACAACTTCAACGGTATCCGCATCAAGACCGACTACCACAGCCTCCTCGATAAGAAAGAAAAAGAGCGCTTCGAGGCCCGCCAGAACTGGACCTCCATCGAGAACATCAAAGAGTTGAAGGCCGGCTACATCTCGCAGGTCGTCCACAAGATCTGCGAGCTGGTCGAGAAGTATGATGCCGTGATCGCTCTCGAGGATCTGAACTCCGGCTTCAAGAACAGCCGCGTCAAGGTTGAGAAGCAGGTCTACCAAAAGTTCGAGAAGATGCTCATCGACAAGCTCAACTACATGGTCGACAAGAAGTCGAACCCCTGCGCCACCGGCGGAGCCCTCAAGGGCTACCAGATCACTAACAAGTTCGAGTCGTTCAAGTCCATGAGCACCCAGAACGGTTTCATCTTCTACATCCCCGCCTGGCTCACCTCCAAGATCGATCCCTCGACCGGCTTCGTGAACCTGCTCAAGACCAAGTACACCTCCATTGCCGATTCCAAGAAGTTCATCTCCAGCTTCGATCGCATCATGTACGTCCCCGAGGAAGATCTCTTCGAGTTCGCCCTGGATTATAAGAACTTCAGCCGCACCGACGCCGATTACATTAAGAAGTGGAAGCTCTACTCCTACGGCAACCGCATCCGCATCTTCCGCAATCCTAAGAAGAACAACGTTTTCGATTGGGAAGAAGTCTGCCTCACCTCCGCCTACAAAGAACTGTTCAACAAGTACGGCATCAACTACCAGCAGGGCGACATCCGCGCTCTGCTCTGCGAGCAGTCCGATAAGGCCTTCTACTCCTCCTTCATGGCCCTCATGTCCCTGATGCTCCAGATGCGCAACTCCATCACCGGCCGGACCGATGTCGATTTCCTCATCTCCCCTGTCAAGAACTCGGATGGTATCTTCTACGATTCCCGGAACTACGAGGCTCAAGAGAACGCCATCTTGCCCAAGAACGCCGACGCCAACGGCGCCTACAATATCGCCCGCAAGGTCCTCTGGGCCATCGGCCAGTTCAAGAAAGCTGAGGACGAGAAGCTCGATAAGGTCAAGATCGCCATTTCCAACAAAGAGTGGCTCGAGTACGCCCAGACCTCCGTCAAGCAC
Protein Sequence
MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAEDYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEIAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSEEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGEFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLSDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNGPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQLQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDLLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSKDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGNYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLIDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVEEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKEELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKINTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDYHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGFKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQNGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDYKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNYEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSVKH
nucleoplasmin NLS — bipartite nuclear localization signal f… 11706..11754 Fwd
DNA Sequence
AAGCGCCCTGCCGCCACCAAGAAGGCTGGCCAGGCAAAGAAGAAGAAA
Protein Sequence
KRPAATKKAGQAKKKK
3xHA — three tandem HA epitope tags 11760..11841 Fwd
DNA Sequence
TACCCCTACGATGTCCCCGATTACGCTTACCCTTACGACGTGCCTGACTACGCCTATCCGTACGACGTCCCGGATTATGCC
Protein Sequence
YPYDVPDYAYPYDVPDYAYPYDVPDYA
SV40 NLS — nuclear localization signal of SV40 (si… 11844..11865 Fwd
DNA Sequence
CCCAAGAAGAAGCGCAAGGTC
Protein Sequence
PKKKRKV
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 859..951 Rev
DNA Sequence
TTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAAT
protein_bind 2
Label Gene Product Position Strand
FRT (minimal) — — 1243..1277 Fwd
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
FRT (minimal) — — 14623..14657 Rev
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
rep_origin 2
Label Gene Product Position Strand
AMA1 — — 1589..6838 Fwd
DNA Sequence
AGCTTATTTTTTGTATACTGTTTTGTGATAGCACGAAGTTTTTCCACGGTATCTTGTTAAAAATATATATTTGTGGCGGGCTTACCTACATCAAATTAATAAGAGACTAATTATAAACTAAACACACAAGCAAGCTACTTTAGGGTAAAAGTTTATAAATGCTTTTGACGTATAAACGTTGCTTGTATTTATTATTACAATTAAAGGTGGATAGAAAACCTAGAGACTAGTTAGAAACTAATCTCAGGTTTGCGTTAAACTAAATCAGAGCCCGAGAGGTTAACAGAACCTAGAAGGGGACTAGATATCCGGGTAGGGAAACAAAAAAAAAAAACAAGACAGCCACATATTAGGGAGACTAGTTAGAAGCTAGTTCCAGGACTAGGAAAATAAAAGACAATGATACCACAGTCTAGTTGACAACTAGATAGATTCTAGATTGAGGCCAAAGTCTCTGAGATCCAGGTTAGTTGCAACTAATACTAGTTAGTATCTAGTCTCCTATAACTCTGAAGCTAGAATAACTTACTACTATTATCCTCACCACTGTTCAGCTGCGCAAACGGAGTGATTGCAAGGTGTTCAGAGACTAGTTATTGACTAGTCAGTGACTAGCAATAACTAACAAGGTATTAACCTACCATGTCTGCCATCACCCTGCACTTCCTCGGGCTCAGCAGCCTTTTCCTCCTCATTTTCATGCTCATTTTCCTTGTTTAAGACTGTGACTAGTCAAAGACTAGTCCAGAACCACAAAGGAGAAATGTCTTACCACTTTCTTCATTGCTTGTCTCTTTTGCATTATCCATGTCTGCAACTAGTTAGAGTCTAGTTAGTGACTAGTCCGACGAGGACTTGCTTGTCTCCGGATTGTTGGAGGAACTCTCCAGGGCCTCAAGATCCACAACAGAGCCTTCTAGAAGACTGGTCAATAACTAGTTGGTCTTTGTCTGAGTCTGACTTACGAGGTTGCATACTCGCTCCCTTTGCCTCGTCAATCGATGAGAAAAAGCGCCAAAACTCGCAATATGGCTTTGAACCACACGGTGCTGAGACTAGTTAGAATCTAGTCCCAAACTAGCTTGGATAGCTTACCTTTGCCCTTTGCGTTGCGACAGGTCTTGCAGGGTATGGTTCCTTTCTCACCAGCTGATTTAGCTGCCTTGCTACCCTCACGGCGGATCTGCATAAAGAGTGGCTAGAGGTTATAAATTAGCACTGATCCTAGGTACGGGGCTGAATGTAACTTGCCYTTCCTTTCTCATCGCGCGGCAAGACAGGCTTGCTCAAATTCCTACCAGTCACAGGGGTATGCACGGCGTACGGACCACTTGAACTAGTCACAGATTAGTTAGCAACTAGTCTGCATTGAATGGCTGTACTTACGGGCCCTCGCCATTGTCCTGATCATTTCCAGCTTCACCCTCGTTGCTGCAAAGTAGTTAGTGACTAGTCAAGGACTAGTTGAAATGGGAGAAGAAACTCACGAATTCTCGACACCCTTAGTATTGTGGTCCTTGGACTTGGTGCTGCTATATATTAGCTAATACACTAGTTAGACTCACAGAAACTTACGCAGCTCGCTTGCKCTTCTTGGTAGGAGTCGGGGTTGGGAGAACAGTGCCTTCAAACAAGCCTTCATACCATGCTRCTTGACTAGTCAGGGACTAGTCACCAAGTAATCTAGATAGGACTTGCCTTTGGCCTCCATCAGTTCCTTCATAGTGGGAGGTCCATTGTGCAATGTAAACTCCATGCCGTGGGAGTTCTTGTCCTTCAAGTGCTTGACCAATATGTTTCTGTTGGCAGAGGGAACCTGTCAACTAGTTAATAACTAGTCAGAAACTAGTATAGCAGTAGACTCACTGTACGCTTGAGGCATCCCTTCACTCGGCAGTAGACTTCATATGGATGGATATCAGGCACGCCATTGTCGTCCTGTGGACTAGTCAGTAACTAGGCTTAAAGCTAGTCGGGTCGGCTTACTATCTTGAAATCCGGCAGCGTAAGCTCCCCGTCCTTAACTGCCTCGAGATAGTGACAGTACTCTGGGGACTTTCGGAGATCGTTATCGCGAATGCTCGGCATACTAATCGTTGACTAGTCTTGGACTAGTCCCGAGCAAAAAGGATTGGAGGAGGAGGAGGAAGGTGAGAGTGAGACAAAGAGCGAAATAAGAGCTTCAAAGGCTATCTCTAAGCAGTATGAAGGTTAAGTATCTAGTTCTTGACTAGATTTAAAAGAGATTTCGACTAGTTATGTACCTGGAGTTTGGATATAGGAATGTGTTGTGGTAACGAAATGTAAGGGGGAGGAAAGAAAAAGTCGGTCAAGAGGTAACTCTAAGTCGGCCATTCCTTTTTGGGAGGCGCTAACCATAAACGGCATGGTCGACTTAGAGTTAGCTCAGGGAATTTAGGGAGTTATCTGCGACCACCGAGGAACGGCGGAATGCCAAAGAATCCCGATGGAGCTCTAGCTGGCGGTTGACAACCCCACCTTTTGGCGTTTCTGCGGCGTTGCAGGCGGGACTGGATACTTCGTAGAACCAGAAAGGCAAGGCAGAACGCGCTCAGCAAGAGTGTTGGAAGTGATAGCATGATGTGCCTTGTTAACTAGGTCAAAATCTGCAGTATGCTTGATGTTATCCAAAGTGTGAGAGAGGAAGGTCCAAACATACACGATTTGGAGAGGGCCTAGGTATAAGAGTTTTTGAGTAGAACGCATGTGAGCCCAGCCATCTCGAGGAGATTAAACACGGGCCGGCATTTGATGGCTATGTTAGTACCCCAATGGAAACGGTGAGAGTCCAGTGGTCGCAGATAACTCCCTAAATTCCCTGAGCTAACTCTAAGTCGACCATGCCGTTTATGGTTAGCGCCTCCCAAAAAGGAATGGCCGACTTAGAGTTACCTCTTGACCGACTTTTTCTTTCCTCCCCCTTACATTTCGTTACCACAACACATTCCTATATCCAAACTCCAGGTACATAACTAGTCGAAATCTCTTTTAAATCTAGTCAAGAACTAGATACTTAACCTTCATACTGCTTAGAGATAGCCTTTGAAGCTCTTATTTCGCTCTTTGTCTCACTCTCACCTTCCTCCTCCTCCTCCAATCCTTTTTGCTCGGGACTAGTCCAAGACTAGTCAACGATTAGTATGCCGAGCATTCGCGATAACGATCTCCGAAAGTCCCCAGAGTACTGTCACTATCTCGAGGCAGTTAAGGACGGGGAGCTTACGCTGCCGGATTTCAAGATAGTAAGCCGACCCGACTAGCTTTAAGCCTAGTTACTGACTAGTCCACAGGACGACAATGGCGTGCCTGATATCCATCCATATGAAGTCTACTGCCGAGTGAAGGGATGCCTCAAGCGTACAGTGAGTCTACTGCTATACTAGTTTCTGACTAGTTATTAACTAGTTGACAGGTTCCCTCTGCCAACAGAAACATATTGGTCAAGCACTTGAAGGACAAGAACTCCCACGGCATGGAGTTTACATTGCACAATGGACCTCCCACTATGAAGGAACTGATGGAGGCCAAAGGCAAGTCCTATCTAGATTACTTGGTGACTAGTCCCTGACTAGTCAAGYAGCATGGTATGAAGGCTTGTTTGAAGGCACTGTTCTCCCAACCCCGACTCCTACCAAGAAGMGCAAGCGAGCTGCGTAAGTTTCTGTGAGTCTAACTAGTGTATTAGCTAATATATAGCAGCACCAAGTCCAAGGACCACAATACTAAGGGTGTCGAGAATTCGTGAGTTTCTTCTCCCATTTCAACTAGTCCTTGACTAGTCACTAACTACTTTGCAGCAACGAGGGTGAAGCTGGAAATGATCAGGACAATGGCGAGGGCCCGTAAGTACAGCCATTCAATGCAGACTAGTTGCTAACTAATCTGTGACTAGTTCAAGTGGTCCGTACGCCGTGCATACCCCTGTGACTGGTAGGAATTTGAGCAAGCCTGTCTTGCCGCGCGATGAGAAAGGAARGGCAAGTTACATTCAGCCCCGTACCTAGGATCAGTGCTAATTTATAACCTCTAGCCACTCTTTATGCAGATCCGCCGTGAGGGTAGCAAGGCAGCTAAATCAGCTGGTGAGAAAGGAACCATACCCTGCAAGACCTGTCGCAACGCAAAGGGCAAAGGTAAGCTATCCAAGCTAGTTTGGGACTAGATTCTAACTAGTCTCAGCACCGTGTGGTTCAAAGCCATATTGCGAGTTTTGGCGCTTTTTCTCATCGATTGACGAGGCAAAGGGAGCGAGTATGCAACCTCGTAAGTCAGACTCAGACAAAGACCAACTAGTTATTGACCAGTCTTCTAGAAGGCTCTGTTGTGGATCTTGAGGCCCTGGAGAGTTCCTCCAACAATCCGGAGACAAGCAAGTCCTCGTCGGACTAGTCACTAACTAGACTCTAACTAGTTGCAGACATGGATAATGCAAAAGAGACAAGCAATGAAGAAAGTGGTAAGACATTTCTCCTTTGTGGTTCTGGACTAGTCTTTGACTAGTCACAGTCTTAAACAAGGAAAATGAGCATGAAAATGAGGAGGAAAAGGCTGCTGAGCCCGAGGAAGTGCAGGGTGATGGCAGACATGGTAGGTTAATACCTTGTTAGTTATTGCTAGTCACTGACTAGTCAATAACTAGTCTCTGAACACCTTGCAATCACTCCGTTTGCGCAGCTGAACAGTGGTGAGGATAATAGTAGTAAGTTATTCTAGCTTCAGAGTTATAGGAGACTAGATACTAACTAGTATTAGTTGCAACTAACCTGGATCTCAGAGACTTTGGCCTCAATCTAGAATCTATCTAGTTGTCAACTAGACTGTGGTATCATTGTCTTTTATTTTCCTAGTCCTGGAACTAGCTTCTAACTAGTCTCCCTAATATGTGGCTGTCTTGTTTTTTTTTTTTGTTTCCCTACCCGGATATCTAGTCCCCTTMTAGGTTCTGTTAACCTCTCGGGCTCTGATTTAGTTTAACGCAAACCTGAGATTAGTTTCTAACTAGTCTCTAGGTTTTCTATCCACCTTTAATTGTAATAATAAATACAAGCAACGTTTATACGTCAAAAGCATTTATAAGCTTTTACCCTAAAGTAGCTTGCTTGTGTGTTTAGTTTATAATTAGTCTCTTATTAATTTGATGTAGGTAAGCCCGCCACAAATATATATTTTTAACAAGATACCGTGGAAAAACTTCGTGCTATCACAAAACAGTATACAAAAAATAAGCT
ori — — 15412..16001 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTAGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA