pAC1430

127981 Synthetic Bacterial Expression

Plasmid Information

Purpose
AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)
Depositor
Uffe Mortensen
Article
Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.
Insert
Cpf1
Tags
SV40 NLS
Mutation
codon optimized for Aspergillus nidulans
Promoter
Aspergillus nidulans tef1 promoter
Available Since
Aug. 19, 2019
Availability
Available

Sequence Information

Length
15,497 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 5
Label Gene Product Position Strand
AmpR bla beta-lactamase 6489..7350 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGTAGCGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCGATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
SV40 NLS — nuclear localization signal of SV40 (si… 10978..10999 Rev
DNA Sequence
CCCAAGAAGAAGCGCAAGGTC
Protein Sequence
PKKKRKV
3xHA — three tandem HA epitope tags 11002..11083 Rev
DNA Sequence
TACCCCTACGATGTCCCCGATTACGCTTACCCTTACGACGTGCCTGACTACGCCTATCCGTACGACGTCCCGGATTATGCC
Protein Sequence
YPYDVPDYAYPYDVPDYAYPYDVPDYA
nucleoplasmin NLS — bipartite nuclear localization signal f… 11089..11137 Rev
DNA Sequence
AAGCGCCCTGCCGCCACCAAGAAGGCTGGCCAGGCAAAGAAGAAGAAA
Protein Sequence
KRPAATKKAGQAKKKK
LbCpf1 — CRISPR-associated protein from Lachnosp… 11137..14821 Rev
DNA Sequence
ATGTCCAAGCTCGAGAAGTTCACCAACTGCTACTCCCTCAGCAAGACCCTCCGCTTCAAGGCTATCCCCGTCGGCAAGACCCAAGAGAATATCGATAACAAGCGCCTCCTCGTCGAGGATGAGAAGCGCGCCGAGGATTACAAGGGCGTCAAGAAGCTCCTCGATCGCTACTACCTCTCCTTCATCAACGATGTCCTCCACTCCATCAAGCTCAAGAACCTCAACAACTACATCTCCCTGTTCCGCAAGAAAACCCGCACCGAGAAAGAGAACAAAGAGCTGGAAAACCTCGAGATCAACCTCCGCAAAGAGATCGCCAAGGCCTTCAAGGGCAACGAGGGCTACAAGTCCCTGTTCAAGAAGGATATCATCGAAACCATCCTGCCTGAGTTCCTCGATGATAAGGATGAGATCGCCCTCGTCAACTCCTTCAACGGCTTCACTACCGCCTTCACCGGTTTCTTCGATAACCGCGAAAACATGTTCTCCGAGGAAGCCAAGTCCACCTCTATCGCCTTCCGCTGCATCAACGAGAACTTGACCCGCTACATCAGCAACATGGATATCTTCGAGAAGGTCGATGCCATCTTCGACAAGCACGAGGTCCAAGAGATCAAAGAAAAGATCCTCAACTCCGATTACGATGTCGAGGATTTCTTCGAGGGCGAGTTCTTCAACTTCGTGCTCACCCAAGAGGGCATCGATGTCTACAACGCCATCATCGGCGGCTTCGTCACCGAGTCCGGCGAGAAGATCAAGGGCCTCAACGAGTACATCAACCTCTACAATCAAAAGACCAAGCAGAAGCTCCCCAAGTTCAAGCCCCTCTACAAGCAGGTCCTCTCCGATCGCGAGTCCCTGTCCTTCTACGGCGAGGGTTACACCTCCGATGAGGAAGTCCTCGAGGTCTTTCGCAACACCCTCAACAAGAACTCCGAGATCTTCTCCAGCATCAAGAAGTTGGAGAAGCTTTTCAAGAACTTCGATGAGTACTCCTCCGCCGGCATCTTCGTCAAGAACGGCCCTGCTATCTCCACCATCTCCAAGGACATCTTCGGCGAGTGGAACGTCATCCGCGATAAGTGGAACGCCGAGTACGATGACATCCACCTCAAGAAGAAGGCCGTCGTTACCGAGAAGTACGAGGATGATCGCCGCAAGTCCTTCAAGAAGATCGGCTCCTTCAGCCTCGAGCAGCTCCAAGAGTACGCCGATGCCGATCTCTCCGTCGTCGAGAAGCTGAAAGAGATCATCATCCAGAAGGTGGACGAGATCTACAAGGTCTACGGCTCCTCTGAGAAGCTGTTCGATGCCGACTTCGTCCTCGAAAAGTCCCTGAAGAAGAACGACGCCGTCGTCGCTATCATGAAGGATCTGCTCGATTCCGTGAAGTCGTTCGAGAACTACATCAAGGCTTTCTTCGGTGAGGGCAAAGAGACTAACCGCGACGAGAGCTTCTACGGTGATTTCGTCCTGGCCTACGATATCCTCCTCAAGGTCGATCACATCTACGATGCTATCCGCAACTACGTCACCCAGAAGCCTTACTCCAAGGATAAGTTCAAGTTGTACTTCCAGAATCCTCAGTTCATGGGCGGCTGGGACAAAGACAAAGAAACCGATTACCGCGCCACCATCCTCCGCTACGGCAGCAAGTACTACCTCGCCATCATGGATAAGAAATACGCCAAGTGCCTCCAAAAGATCGATAAGGACGATGTCAACGGCAACTACGAAAAGATCAACTACAAGCTGCTCCCCGGTCCTAACAAGATGCTGCCCAAGGTGTTCTTCAGCAAGAAATGGATGGCCTACTACAACCCCTCCGAGGATATCCAGAAGATCTATAAGAACGGCACCTTCAAAAAGGGCGATATGTTCAACCTCAACGATTGCCACAAGCTCATCGATTTCTTCAAGGATTCCATCTCGCGCTACCCCAAGTGGTCCAACGCCTACGATTTCAACTTCTCCGAGACTGAGAAGTATAAGGATATCGCTGGCTTCTACCGTGAGGTCGAGGAACAGGGTTACAAGGTGTCCTTCGAGTCCGCCTCCAAGAAAGAGGTCGATAAGCTCGTCGAAGAGGGCAAGCTCTACATGTTCCAAATCTACAACAAGGATTTCTCCGATAAGTCCCACGGCACCCCTAACCTCCACACCATGTACTTCAAGCTGCTGTTCGACGAGAACAACCACGGCCAGATCCGCCTCTCCGGCGGTGCCGAGCTGTTCATGCGCCGTGCTAGCCTGAAGAAAGAAGAGTTGGTCGTCCATCCTGCTAACTCCCCGATCGCCAACAAGAATCCTGATAACCCCAAGAAAACTACCACCTTGTCCTACGACGTCTACAAGGACAAGCGCTTCAGCGAGGATCAGTACGAGCTGCACATCCCTATCGCCATCAACAAGTGCCCTAAGAACATCTTCAAGATCAATACCGAGGTCCGCGTCCTCCTGAAGCACGATGACAACCCCTACGTCATCGGTATCGATCGCGGCGAGCGCAACCTCCTCTACATCGTCGTCGTCGATGGCAAGGGTAACATCGTCGAGCAGTACTCCCTGAACGAAATCATCAACAACTTCAACGGTATCCGCATCAAGACCGACTACCACAGCCTCCTCGATAAGAAAGAAAAAGAGCGCTTCGAGGCCCGCCAGAACTGGACCTCCATCGAGAACATCAAAGAGTTGAAGGCCGGCTACATCTCGCAGGTCGTCCACAAGATCTGCGAGCTGGTCGAGAAGTATGATGCCGTGATCGCTCTCGAGGATCTGAACTCCGGCTTCAAGAACAGCCGCGTCAAGGTTGAGAAGCAGGTCTACCAAAAGTTCGAGAAGATGCTCATCGACAAGCTCAACTACATGGTCGACAAGAAGTCGAACCCCTGCGCCACCGGCGGAGCCCTCAAGGGCTACCAGATCACTAACAAGTTCGAGTCGTTCAAGTCCATGAGCACCCAGAACGGTTTCATCTTCTACATCCCCGCCTGGCTCACCTCCAAGATCGATCCCTCGACCGGCTTCGTGAACCTGCTCAAGACCAAGTACACCTCCATTGCCGATTCCAAGAAGTTCATCTCCAGCTTCGATCGCATCATGTACGTCCCCGAGGAAGATCTCTTCGAGTTCGCCCTGGATTATAAGAACTTCAGCCGCACCGACGCCGATTACATTAAGAAGTGGAAGCTCTACTCCTACGGCAACCGCATCCGCATCTTCCGCAATCCTAAGAAGAACAACGTTTTCGATTGGGAAGAAGTCTGCCTCACCTCCGCCTACAAAGAACTGTTCAACAAGTACGGCATCAACTACCAGCAGGGCGACATCCGCGCTCTGCTCTGCGAGCAGTCCGATAAGGCCTTCTACTCCTCCTTCATGGCCCTCATGTCCCTGATGCTCCAGATGCGCAACTCCATCACCGGCCGGACCGATGTCGATTTCCTCATCTCCCCTGTCAAGAACTCGGATGGTATCTTCTACGATTCCCGGAACTACGAGGCTCAAGAGAACGCCATCTTGCCCAAGAACGCCGACGCCAACGGCGCCTACAATATCGCCCGCAAGGTCCTCTGGGCCATCGGCCAGTTCAAGAAAGCTGAGGACGAGAAGCTCGATAAGGTCAAGATCGCCATTTCCAACAAAGAGTGGCTCGAGTACGCCCAGACCTCCGTCAAGCAC
Protein Sequence
MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAEDYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEIAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSEEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGEFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLSDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNGPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQLQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDLLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSKDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGNYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLIDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVEEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKEELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKINTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDYHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGFKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQNGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDYKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNYEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSVKH
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 6397..6489 Fwd
DNA Sequence
TTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAAT
protein_bind 2
Label Gene Product Position Strand
FRT (minimal) — — 6071..6105 Rev
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
FRT (minimal) — — 8815..8849 Fwd
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
rep_origin 2
Label Gene Product Position Strand
AMA1 — — 508..5759 Fwd
DNA Sequence
AGCTTATTTTTTGTATACTGTTTTGTGATAGCACGAAGTTTTTCCACGGTATCTTGTTAAAAATATATATTTGTGGCGGGCTTACCTACATCAAATTAATAAGAGACTAATTATAAACTAAACACACAAGCAAGCTACTTTAGGGTAAAAGTTTATAAATGCTTTTGACGTATAAACGTTGCTTGTATTTATTATTACAATTAAAGGTGGATAGAAAACCTAGAGACTAGTTAGAAACTAATCTCAGGTTTGCGTTAAACTAAATCAGAGCCCGAGAGGTTAACAGAACCTAGAAGGGGACTAGATATCCGGGTAGGGAAACNAAAAAAAAAAAACAAGACAGCCACATATTAGGGAGACTAGTTAGAAGCTAGTTCCAGGACTAGGAAAATAAAAGACAATGATACCACAGTCTAGTTGACAACTAGATAGATTCTAGATTGAGGCCAAAGTCTCTGAGATCCAGGTTAGTTGCAACTAATACTAGTTAGTATCTAGTCTCCTATAACTCTGAAGCTAGAATAACTTACTACTATTATCCTCACCACTGTTCAGCTGCGCAAACGGAGTGATTGCAAGGTGTTCAGAGACTAGTTATTGACTAGTCAGTGACTAGCAATAACTAACAAGGTATTAACCTACCATGTCTGCCATCACCCTGCACTTCCTCGGGCTCAGCAGCCTTTTCCTCCTCATTTTCATGCTCATTTTCCTTGTTTAAGACTGTGACTAGTCAAAGACTAGTCCAGAACCACAAAGGAGAAATGTCTTACCACTTTCTTCATTGCTTGTCTCTTTTGCATTATCCATGTCTGCAACTAGTTAGAGTCTAGTTAGTGACTAGTCCGACGAGGACTTGCTTGTCTCCGGATTGTTGGAGGAACTCTCCAGGGCCTCAAGATCCACAACAGAGCCTTCTAGAAGACTGGTCAATAACTAGTTGGTCTTTGTCTGAGTCTGACTTACGAGGTTGCATACTCGCTCCCTTTGCCTCGTCAATCGATGAGAAAAAGCGCCAAAACTCGCAATATGGCTTTGAACCACACGGTGCTGAGACTAGTTAGAATCTAGTCCCAAACTAGCTTGGATAGCTTACCTTTGCCCTTTGCGTTGCGACAGGTCTTGCAGGGTATGGTTCCTTTCTCACCAGCTGATTTAGCTGCCTTGCTACMCTCACGGCGGATCTGCATAAAGAGTGGCTAGAGGTTATAAATTAGCACTGATCCTAGGTACGGGGCTGAATGTAACTTGCCYTTCCTTTCTCATCGCGCGGCAAGACAGGCTTGCTCAAATTCCTACCAGTCACAGGGGTATGCACGGCGTACGGACCACTTGAACTAGTCACAGATTAGTTAGCAACTAGTCTGCATTGAATGGCTGTACTTACGGGCCCTCGCCATTGTCCTGATCATTTCCAGCTTCACCCTCGTTGCTGCAAAGTAGTTAGTGACTAGTCAAGGACTAGTTGAAATGGGAGAAGAAACTCACGAATTCTCGACACCCTTAGTATTGTGGTCCTTGGACTTGGTGCTGCTATATATTAGCTAATACACTAGTTAGACTCACAGAAACTTACGCAGCTCGCTTGCGCTTCTTGGTAGGAGTCGGGGTTGGGAGAACAGTGCCTTCAAACAAGCCTTCATACCATGCTACTTGACTAGTCAGGGACTAGTCACCAAGTAATCTAGATAGGACTTKCCTTTGGCCTCCATCAGTTCCTTCATAGTGGGAGGTCCATTGTGCAATGTAAACTCCATGCCGTGGGAGTTCTTGTCCTTCAAGTGCTTGACCAATATGTTTCTGTTGGCAGAGGGAACCTGTCAACTAGTTAATAACTAGTCAGAAACTAGTATAGCAGTAGACTCACTGTACGCTTGAGGCATCCCTTCACTCGGCAGTAGACTTCATATGGATGGATATCAGGCACGCCATTGTCGTCCTGTGGACTAGTCAGTAACTAGGCTTAAAGCTAGTCGGGTCGGCTTACTATCTTGAAATCCGGCAGCGTAAGCTCCCCGTCCTTAACTGCCTCGAGATAGTGACAGTACTCTGGGGACTTTCGGAGATCGTTATCGCGAATGCTCGGCATACTAATCGTTGACTAGTCTTGGACTAGTCCCGAGCAAAAAGGATTGGAGGAGGAGGAGGAAGGTGAGAGTGAGACAAAGAGCGAAATAAGAGCTTCAAAGGCTATCTCTAAGCAGTATGAAGGTTAAGTATCTAGTTCTTGACTAGATTTAAAAGAGATTTCGACTAGTTATGTACCTGGAGTTTGGATATAGGAATGTGTTGTGGTAACGAAATGTAAGGGGGAGGAAAGAAAAAGTCGGTCAAGAGGTAACTCTAAGTCGGCCATTCCTTTTTGGGAGGCGCTAACCATAAACGGCATGGTCGACTTAGAGTTAGCTCAGGGAATTTAGGGAGTTATCTGCGACCACTGGACTCTCACCGTTTCCATTGGGGTACTAACATAGCCATCAAATGCCGGCCCGTGTTTAATCTCCTCGAGATGGCTGGGCTCACATGCGTTCTACTCAAAAACTCTTATACCTAGGCCCTCTCCCAATCGTGTATGTTTGGACCTTCCTCTCTCACACTTTGGATAACATCAAGCATACTGCAGATTTTGACCTAGTTAACAAGGCACATCATGCTATCACTTCCAACACTCTTGCTGAGCGCGTTCTGCCTTGCCTTTCTGGTTCTACGAAGTATCCAGTCCCGCCTGCAACGCCGCAGAAACGCCAAAAGGTGGGGTTGTCAACCGCCAGCTAGAGCTCCATCGGGATTCTTTGGCATTCAGCCGTTCCTCGGTGGTCGCAGATAACTCCCTAAATTCCCTGAGCTAACTCTAAGTCGACCATGCCGTTTATGGTTAGCGCCTCCCAAAAAGGAATGGCCGACTTAGAGTTACCTCTTGACCGACTTTTTCTTTCCTCCCCCTTACATTTCGTTACCACAACACATTCCTATATCCAAACTCCAGGTACATAACTAGTCGAAATCTCTTTTAAATCTAGTCAAGAACTAGATACTTAACCTTCATACTGCTTAGAGATAGCCTTTGAAGCTCTTATTTCGCTCTTTGTCTCACTCTCACCTTCCTCCTCCTCCTCCAATCCTTTTTGCTCGGGACTAGTCCAAGACTAGTCAACGATTAGTATGCCGAGCATTCGCGATAACGATCTCCGAAAGTCCCCAGAGTACTGTCACTATCTCGAGGCAGTTAAGGACGGGGAGCTTACGCTGCCGGATTTCAAGATAGTAAGCCGACCCGACTAGCTTTAAGCCTAGTTACTGACTAGTCCACAGGACGACAATGGCGTGCCTGATATCCATCMATATGAAGTCTACTGCCGAGTGAAGGGATGCCTCAAGCGTACAGTGAGTCTACTGCTATACTAGTTTCTGACTAGTTATTAACTAGTTGACAGGTTCCCTCTGCCAACAGAAACATATTGGTCAAGCACTTGAAGGACAAGAACTCCCACGGCATGGAGTTTACATTGCACAATGGACCTCCCACTATGAAGGAACTGATGGAGGCCAAAGGMAAGTCCTATCTAGATTACTTGGTGACTAGTCCCTGACTAGTCAAGTAGCATGGTATGAAGGCTTGTTTGAAGGCACTGTTCTCCCAACCCCGACTCCTACCAAGAAGCGCAAGCGAGCTGCGTAAGTTTCTGTGAGTCTAACTAGTGTATTAGCTAATATATAGCAGCACCAAGTCCAAGGACCACAATACTAAGGGTGTCGAGAATTCGTGAGTTTCTTCTCCCATTTCAACTAGTCCTTGACTAGTCACTAACTACTTTGCAGCAACGAGGGTGAAGCTGGAAATGATCAGGACAATGGCGAGGGCCCGTAAGTACAGCCATTCAATGCAGACTAGTTGCTAACTAATCTGTGACTAGTTCAAGTGGTCCGTACGCCGTGCATACCCCTGTGACTGGTAGGAATTTGAGCAAGCCTGTCTTGCCGCGCGATGAGAAAGGAARGGCAAGTTACATTCAGCCCCGTACCTAGGATCAGTGCTAATTTATAACCTCTAGCCACTCTTTATGCAGATCCGCCGTGAGKGTAGCAAGGCAGCTAAATCAGCTGGTGAGAAAGGAACCATACCCTGCAAGACCTGTCGCAACGCAAAGGGCAAAGGTAAGCTATCCAAGCTAGTTTGGGACTAGATTCTAACTAGTCTCAGCACCGTGTGGTTCAAAGCCATATTGCGAGTTTTGGCGCTTTTTCTCATCGATTGACGAGGCAAAGGGAGCGAGTATGCAACCTCGTAAGTCAGACTCAGACAAAGACCAACTAGTTATTGACCAGTCTTCTAGAAGGCTCTGTTGTGGATCTTGAGGCCCTGGAGAGTTCCTCCAACAATCCGGAGACAAGCAAGTCCTCGTCGGACTAGTCACTAACTAGACTCTAACTAGTTGCAGACATGGATAATGCAAAAGAGACAAGCAATGAAGAAAGTGGTAAGACATTTCTCCTTTGTGGTTCTGGACTAGTCTTTGACTAGTCACAGTCTTAAACAAGGAAAATGAGCATGAAAATGAGGAGGAAAAGGCTGCTGAGCCCGAGGAAGTGCAGGGTGATGGCAGACATGGTAGGTTAATACCTTGTTAGTTATTGCTAGTCACTGACTAGTCAATAACTAGTCTCTGAACACCTTGCAATCACTCCGTTTGCGCAGCTGAACAGTGGTGAGGATAATAGTAGTAAGTTATTCTAGCTTCAGAGTTATAGGAGACTAGATACTAACTAGTATTAGTTGCAACTAACCTGGATCTCAGAGACTTTGGCCTCAATCTAGAATCTATCTAGTTGTCAACTAGACTGTGGTATCATTGTCTTTTATTTTCCTAGTCCTGGAACTAGCTTCTAACTAGTCTCCCTAATATGTGGCTGTCTTGTTTTTTTTTTTTTGTTTCCCTACCCGGATATCTAGTCCCCTTCTAGGTTCTGTTAACCTCTCGGGCTCTGATTTAGTTTAACGCAAACCTGAGATTAGTTTCTAACTAGTCTCTAGGTTTTCTATCCACCTTTAATTGTAATAATAAATACAAGCAACGTTTATACGTCAAAAGCATTTATAAACTTTTACCCTAAAGTAGCTTGCTTGTGTGTTTAGTTTATAATTAGTCTCTTATTAATTTGATGTAGGTAAGCCCGCCACAAATATATATTTTTAACAAGATACCGTGGAAAAACTTCGTGCTATCACAAAACAGTATACAAAAAATAAGCT
ori — — 7471..8060 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA