Ubiquitin I44A

12648 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Rachel Klevit
Article
Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.
Insert
Ubiquitin (UBC Human)
Mutation
Ile44Ala
Available Since
July 31, 2007
Availability
Available

Sequence Information

Length
903 bp
Topology
linear
Molecule Type
genomic DNA

Features

CDS 3
Label Gene Product Position Strand
ubiquitin Homo sapiens UBC human ubiquitin 129..357 Fwd
DNA Sequence
ATGCAGATCTTCGTCAAGACGTTAACCGGTAAAACCATAACTCTGGAAGTTGAACCATCCGATACCATCGAAAACGTTAAGGCTAAAATTCAAGACAAGGAAGGAATTCCACCTGATCAACAAAGATTGGCATTTGCCGGTAAGCAGCTCGAGGACGGTAGAACGCTGTCTGATTACAACATTCAGAAGGAGTCGACCTTACATCTTGTCTTAAGACTAAGAGGTGGT
Protein Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLAFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
6xHis 6xHis affinity tag 383..401 Fwd
DNA Sequence
CATCATCATCATCATCAC
Protein Sequence
HHHHHH
thrombin site thrombin recognition and cleavage site 410..428 Fwd
DNA Sequence
CTGGTGCCGCGCGGCAGC
Protein Sequence
LVPRGS
promoter 2
Label Gene Product Position Strand
T7 promoter 42..61 Fwd
DNA Sequence
TAATACGACTCACTATAGG
tet promoter 735..764 Rev
DNA Sequence
TTGACAGCTTATCATCGATAAGCTTTAAT
protein_bind 1
Label Gene Product Position Strand
lac operator 61..86 Fwd
DNA Sequence
GGAATTGTGAGCGGATAACAATTCC
terminator 1
Label Gene Product Position Strand
T7 terminator 513..561 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG