pET20b-pIgR D1-H6

12530 Homo sapiens Bacterial Expression

Plasmid Information

Depositor
Pamela Bjorkman
Article
Hamburger et al Structure. 2004 Nov . 12(11):1925-35.
Insert
human polymeric immunoglobulin receptor (PIGR Human)
Tags
His
Mutation
Lys 1-Val 109
Available Since
Sept. 1, 2006
Availability
Available

Sequence Information

Length
728 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 1
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 362..380 Fwd
DNA Sequence
CACCACCACCACCACCAC
Protein Sequence
HHHHHH
terminator 1
Label Gene Product Position Strand
T7 terminator — — 446..494 Fwd
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG