6975 pPROEX-HTb-SIS1

1233 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Susan Lindquist
Article
Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep
Insert
sis1 (SIS1 Budding Yeast)
Tags
his tag (tev cleavable)
Available Since
July 18, 2005
Availability
Available

Sequence Information

Length
1,174 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 6..24 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 45..66 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG