pAT28-proD/SaEGFP

120321 Synthetic Bacterial Expression

Plasmid Information

Purpose
Bacterial eGFP labeling
Depositor
James Kirby
Article
Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0
Insert
eGFP with Staphylococcus aureus codon optimization
Promoter
proD
Available Since
Feb. 27, 2020
Availability
Available

Sequence Information

Length
7,499 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
EGFP — the original enhanced GFP (Yang et al.,… 385..1102 Fwd
DNA Sequence
ATGGTTAGTAAAGGAGAAGAATTATTTACAGGTGTAGTTCCAATATTAGTTGAATTAGATGGTGACGTTAATGGTCACAAATTTTCAGTTTCAGGTGAAGGTGAAGGTGATGCGACTTATGGAAAACTAACATTAAAGTTTATTTGTACAACTGGAAAATTACCTGTACCGTGGCCGACATTAGTGACTACACTTACATATGGTGTGCAATGCTTCTCACGTTATCCAGATCATATGAAGCAACATGATTTTTTTAAATCTGCAATGCCTGAGGGATATGTTCAAGAAAGAACTATCTTTTTTAAAGATGATGGAAATTATAAAACTCGTGCGGAGGTTAAGTTCGAAGGTGACACGTTAGTAAATCGTATCGAATTAAAAGGTATTGATTTCAAAGAAGACGGCAATATTTTAGGTCACAAATTAGAATACAATTATAATTCACACAATGTTTATATTATGGCAGACAAACAAAAAAACGGTATTAAGGTGAATTTTAAAATACGTCATAATATCGAAGATGGTTCTGTGCAACTAGCAGATCATTATCAACAGAATACTCCAATTGGTGATGGTCCAGTGTTATTACCTGACAATCATTATTTAAGTACTCAGTCAGCATTAAGTAAAGACCCAAATGAGAAGCGTGATCACATGGTTTTATTAGAATTTGTTACAGCAGCAGGTATTACTCTTGGTATGGATGAATTATATAAA
Protein Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
traJ traJ oriT-recognizing protein 1474..1846 Rev
DNA Sequence
ATGGCTGATGAAACCAAGCCAACCAGGAAGGGCAGCCCACCTATCAAGGTGTACTGCCTTCCAGACGAACGAAGAGCGATTGAGGAAAAGGCGGCGGCGGCCGGCATGAGCCTGTCGGCCTACCTGCTGGCCGTCGGCCAGGGCTACAAAATCACGGGCGTCGTGGACTATGAGCACGTCCGCGAGCTGGCCCGCATCAATGGCGACCTGGGCCGCCTGGGCGGCCTGCTGAAACTCTGGCTCACCGACGACCCGCGCACGGCGCGGTTCGGTGATGCCACGATCCTCGCCCTGCTGGCGAAGATCGAAGAGAAGCAGGACGAGCTTGGCAAGGTCATGATGGGCGTGGTCCGCCCGAGGGCAGAGCCATGA
Protein Sequence
MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAVGQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEEKQDELGKVMMGVVRPRAEP
promoter 1
Label Gene Product Position Strand
lac promoter — — 130..161 Fwd
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
protein_bind 2
Label Gene Product Position Strand
CAP binding site — — 94..116 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator — — 168..185 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
rep_origin 1
Label Gene Product Position Strand
ori — — 6717..7306 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA