RNQ pROEX-HTb

1176 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Susan Lindquist
Article
Sondheimer et al Mol Cell 2000 Jan;5(1):163-72.
Insert
RNQ1 (RNQ1 Budding Yeast)
Tags
6xHis and TEV cleavage site
Available Since
May 25, 2005
Availability
Available

Sequence Information

Length
979 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 138..156 Fwd
DNA Sequence
CATCACCATCACCATCAC
Protein Sequence
HHHHHH
TEV site — tobacco etch virus (TEV) protease recog… 177..198 Fwd
DNA Sequence
GAAAACCTGTATTTTCAGGGC
Protein Sequence
ENLYFQG
protein_bind 1
Label Gene Product Position Strand
lac operator — — 89..106 Fwd
DNA Sequence
TTGTGAGCGGATAACAA