| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| dCas9 | — | catalytically dead mutant of the Cas9 e… | 665..4772 | Fwd | |
|
DNA Sequence
ATGGACAAGAAGTACTCCATCGGCCTGGCGATCGGCACCAACTCCGTCGGCTGGGCCGTCATCACGGACGAGTACAAGGTCCCGTCGAAGAAGTTCAAGGTGCTGGGCAACACGGACCGCCACAGCATCAAGAAGAACCTCATCGGCGCCCTCCTGTTCGACAGCGGCGAGACGGCCGAGGCGACCCGCCTGAAGCGCACGGCGCGGCGGCGGTACACGCGCCGCAAGAACCGCATCTGCTACCTCCAGGAGATCTTCTCCAACGAGATGGCGAAGGTCGACGACTCGTTCTTCCACCGGCTGGAGGAGTCCTTCCTGGTCGAGGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCGACCATCTACCACCTCCGGAAGAAGCTGGTGGACTCGACCGACAAGGCCGACCTGCGCCTGATCTACCTGGCCCTCGCCCACATGATCAAGTTCCGCGGCCACTTCCTCATCGAGGGCGACCTGAACCCGGACAACTCGGACGTCGACAAGCTGTTCATCCAGCTCGTGCAGACCTACAACCAGCTCTTCGAGGAGAACCCGATCAACGCCTCCGGCGTGGACGCGAAGGCCATCCTGTCGGCCCGCCTCTCGAAGTCCCGGCGGCTCGAGAACCTGATCGCCCAGCTGCCGGGCGAGAAGAAGAACGGCCTCTTCGGCAACCTGATCGCGCTCTCCCTGGGCCTGACGCCGAACTTCAAGTCCAACTTCGACCTCGCCGAGGACGCCAAGCTCCAGCTGTCCAAGGACACGTACGACGACGACCTGGACAACCTCCTGGCGCAGATCGGCGACCAGTACGCGGACCTGTTCCTCGCGGCGAAGAACCTCTCCGACGCGATCCTGCTCAGCGACATCCTCCGCGTCAACACCGAGATCACCAAGGCGCCCCTGTCCGCGAGCATGATCAAGCGCTACGACGAGCACCACCAGGACCTCACCCTGCTGAAGGCCCTGGTCCGCCAGCAGCTCCCGGAGAAGTACAAGGAGATCTTCTTCGACCAGTCCAAGAACGGCTACGCGGGCTACATCGACGGCGGCGCGTCCCAGGAGGAGTTCTACAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTCAAGCTGAACCGGGAGGACCTCCTGCGCAAGCAGCGCACGTTCGACAACGGCTCCATCCCCCACCAGATCCACCTGGGCGAGCTGCACGCCATCCTCCGGCGCCAGGAGGACTTCTACCCGTTCCTCAAGGACAACCGCGAGAAGATCGAGAAGATCCTCACGTTCCGGATCCCGTACTACGTCGGCCCCCTCGCCCGCGGCAACTCCCGCTTCGCGTGGATGACGCGCAAGTCGGAGGAGACGATCACCCCGTGGAACTTCGAGGAGGTGGTGGACAAGGGCGCCTCCGCGCAGTCGTTCATCGAGCGGATGACCAACTTCGACAAGAACCTGCCGAACGAGAAGGTGCTGCCCAAGCACAGCCTCCTGTACGAGTACTTCACGGTCTACAACGAGCTCACCAAGGTGAAGTACGTGACGGAGGGCATGCGGAAGCCGGCGTTCCTGTCCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACGAACCGGAAGGTCACCGTCAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACTCGGTCGAGATCAGCGGCGTGGAGGACCGCTTCAACGCGTCGCTGGGCACGTACCACGACCTCCTGAAGATCATCAAGGACAAGGACTTCCTCGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTGCTGACCCTGACGCTCTTCGAGGACCGGGAGATGATCGAGGAGCGCCTCAAGACCTACGCGCACCTGTTCGACGACAAGGTCATGAAGCAGCTCAAGCGGCGGCGGTACACCGGCTGGGGCCGGCTCTCCCGCAAGCTCATCAACGGCATCCGGGACAAGCAGTCGGGCAAGACCATCCTCGACTTCCTGAAGTCCGACGGCTTCGCCAACCGCAACTTCATGCAGCTGATCCACGACGACAGCCTGACGTTCAAGGAGGACATCCAGAAGGCGCAGGTGTCGGGCCAGGGCGACTCCCTGCACGAGCACATCGCGAACCTGGCCGGCAGCCCGGCCATCAAGAAGGGCATCCTGCAGACGGTCAAGGTGGTCGACGAGCTGGTGAAGGTGATGGGCCGCCACAAGCCCGAGAACATCGTCATCGAGATGGCCCGGGAGAACCAGACCACGCAGAAGGGCCAGAAGAACAGCCGCGAGCGCATGAAGCGGATCGAGGAGGGCATCAAGGAGCTCGGCTCGCAGATCCTCAAGGAGCACCCCGTCGAGAACACCCAGCTCCAGAACGAGAAGCTCTACCTCTACTACCTGCAGAACGGCCGGGACATGTACGTGGACCAGGAGCTCGACATCAACCGGCTGTCGGACTACGACGTGGACGCCATCGTCCCGCAGTCGTTCCTGAAGGACGACAGCATCGACAACAAGGTCCTCACCCGCTCGGACAAGAACCGGGGCAAGTCCGACAACGTCCCGTCGGAGGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAGCTCCTCAACGCCAAGCTCATCACGCAGCGCAAGTTCGACAACCTGACGAAGGCGGAGCGGGGCGGCCTCAGCGAGCTCGACAAGGCGGGCTTCATCAAGCGGCAGCTGGTCGAGACGCGGCAGATCACGAAGCACGTGGCCCAGATCCTGGACTCGCGCATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGCGAGGTCAAGGTCATCACCCTCAAGTCCAAGCTGGTGAGCGACTTCCGCAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACGACGCGTACCTGAACGCGGTGGTGGGCACGGCGCTGATCAAGAAGTACCCGAAGCTCGAGAGCGAGTTCGTCTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCGAAGAGCGAGCAGGAGATCGGCAAGGCCACGGCGAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCGAACGGCGAGATCCGCAAGCGCCCGCTCATCGAGACCAACGGCGAGACGGGCGAGATCGTGTGGGACAAGGGCCGCGACTTCGCGACCGTCCGGAAGGTGCTGTCCATGCCCCAGGTCAACATCGTCAAGAAGACGGAGGTCCAGACCGGCGGCTTCAGCAAGGAGTCCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCCAAGAAGTACGGCGGCTTCGACTCCCCCACCGTCGCCTACTCGGTCCTCGTGGTCGCCAAGGTGGAGAAGGGCAAGAGCAAGAAGCTGAAGTCCGTGAAGGAGCTGCTGGGCATCACCATCATGGAGCGGTCGTCGTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTGAAGAAGGACCTCATCATCAAGCTCCCCAAGTACTCCCTGTTCGAGCTGGAGAACGGCCGGAAGCGCATGCTCGCCAGCGCGGGCGAGCTGCAGAAGGGCAACGAGCTGGCGCTGCCGAGCAAGTACGTCAACTTCCTGTACCTCGCCTCGCACTACGAGAAGCTGAAGGGCTCGCCGGAGGACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTCGACGAGATCATCGAGCAGATCTCCGAGTTCTCCAAGCGCGTCATCCTGGCCGACGCGAACCTGGACAAGGTCCTGTCCGCGTACAACAAGCACCGCGACAAGCCGATCCGGGAGCAGGCCGAGAACATCATCCACCTGTTCACGCTGACCAACCTCGGCGCCCCCGCGGCCTTCAAGTACTTCGACACCACGATCGACCGGAAGCGGTACACGAGCACGAAGGAGGTGCTCGACGCCACCCTGATCCACCAGAGCATCACGGGCCTGTACGAGACCCGCATCGACCTCTCCCAGCTGGGCGGCGACTGA
Protein Sequence
MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
|
|||||
| ApmR | aac(3)IV | aminoglycoside 3-N-acetyltransferase ty… | 6386..7187 | Fwd | |
|
DNA Sequence
ATGTCATCAGCGGTGGAGTGCAATGTCGTGCAATACGAATGGCGAAAAGCCGAGCTCATCGGTCAGCTTCTCAACCTTGGGGTTACCCCCGGCGGTGTGCTGCTGGTCCACAGCTCCTTCCGTAGCGTCCGGCCCCTCGAAGATGGGCCACTTGGACTGATCGAGGCCCTGCGTGCTGCGCTGGGTCCGGGAGGGACGCTCGTCATGCCCTCGTGGTCAGGTCTGGACGACGAGCCGTTCGATCCTGCCACGTCGCCCGTTACACCGGACCTTGGAGTTGTCTCTGACACATTCTGGCGCCTGCCAAATGTAAAGCGCAGCGCCCATCCATTTGCCTTTGCGGCAGCGGGGCCACAGGCAGAGCAGATCATCTCTGATCCATTGCCCCTGCCACCTCACTCGCCTGCAAGCCCGGTCGCCCGTGTCCATGAACTCGATGGGCAGGTACTTCTCCTCGGCGTGGGACACGATGCCAACACGACGCTGCATCTTGCCGAGTTGATGGCAAAGGTTCCCTATGGGGTGCCGAGACACTGCACCATTCTTCAGGATGGCAAGTTGGTACGCGTCGATTATCTCGAGAATGACCACTGCTGTGAGCGCTTTGCCTTGGCGGACAGGTGGCTCAAGGAGAAGAGCCTTCAGAAGGAAGGTCCAGTCGGTCATGCCTTTGCTCGGTTGATCCGCTCCCGCGACATTGTGGCGACAGCCCTGGGTCAACTGGGCCGAGATCCGTTGATCTTCCTGCATCCGCCAGAGGCGGGATGCGAAGAATGCGATGCCGCTCGCCAGTCGATTGGC
Protein Sequence
MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRPLEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNVKRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLHLAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGHAFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG
|
|||||
| traJ | traJ | oriT-recognizing protein | 7620..7992 | Rev | |
|
DNA Sequence
ATGGCTGATGAAACCAAGCCAACCAGGAAGGGCAGCCCACCTATCAAGGTGTACTGCCTTCCAGACGAACGAAGAGCGATTGAGGAAAAGGCGGCGGCGGCCGGCATGAGCCTGTCGGCCTACCTGCTGGCCGTCGGCCAGGGCTACAAAATCACGGGCGTCGTGGACTATGAGCACGTCCGCGAGCTGGCCCGCATCAATGGCGACCTGGGCCGCCTGGGCGGCCTGCTGAAACTCTGGCTCACCGACGACCCGCGCACGGCGCGGTTCGGTGATGCCACGATCCTCGCCCTGCTGGCGAAGATCGAAGAGAAGCAGGACGAGCTTGGCAAGGTCATGATGGGCGTGGTCCGCCCGAGGGCAGAGCCATGA
Protein Sequence
MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAVGQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEEKQDELGKVMMGVVRPRAEP
|
|||||
| Factor Xa site | — | Factor Xa recognition and cleavage site | 8371..8383 | Rev | |
|
DNA Sequence
ATAGAGGGGCGT
Protein Sequence
IEGR
|
|||||
| phage phi-C31 integrase | — | integrase from phage phi-C31 | 8562..10380 | Fwd | |
|
DNA Sequence
GTGGACACGTACGCGGGTGCTTACGACCGTCAGTCGCGCGAGCGCGAAAATTCGAGCGCAGCAAGCCCAGCGACACAGCGTAGCGCCAACGAAGACAAGGCGGCCGACCTTCAGCGCGAAGTCGAGCGCGACGGGGGCCGGTTCAGGTTCGTCGGGCATTTCAGCGAAGCGCCGGGCACGTCGGCGTTCGGGACGGCGGAGCGCCCGGAGTTCGAACGCATCCTGAACGAATGCCGCGCCGGGCGGCTCAACATGATCATTGTCTATGACGTGTCGCGCTTCTCGCGCCTGAAGGTCATGGACGCGATTCCGATTGTCTCGGAATTGCTCGCCCTGGGCGTGACGATTGTTTCCACTCAGGAAGGCGTCTTCCGGCAGGGAAACGTCATGGACCTGATTCACCTGATTATGCGGCTCGACGCGTCGCACAAAGAATCTTCGCTGAAGTCGGCGAAGATTCTCGACACGAAGAACCTTCAGCGCGAATTGGGCGGGTACGTCGGCGGGAAGGCGCCTTACGGCTTCGAGCTTGTTTCGGAGACGAAGGAGATCACGCGCAACGGCCGAATGGTCAATGTCGTCATCAACAAGCTTGCGCACTCGACCACTCCCCTTACCGGACCCTTCGAGTTCGAGCCCGACGTAATCCGGTGGTGGTGGCGTGAGATCAAGACGCACAAACACCTTCCCTTCAAGCCGGGCAGTCAAGCCGCCATTCACCCGGGCAGCATCACGGGGCTTTGTAAGCGCATGGACGCTGACGCCGTGCCGACCCGGGGCGAGACGATTGGGAAGAAGACCGCTTCAAGCGCCTGGGACCCGGCAACCGTTATGCGAATCCTTCGGGACCCGCGTATTGCGGGCTTCGCCGCTGAGGTGATCTACAAGAAGAAGCCGGACGGCACGCCGACCACGAAGATTGAGGGTTACCGCATTCAGCGCGACCCGATCACGCTCCGGCCGGTCGAGCTTGATTGCGGACCGATCATCGAGCCCGCTGAGTGGTATGAGCTTCAGGCGTGGTTGGACGGCAGGGGGCGCGGCAAGGGGCTTTCCCGGGGGCAAGCCATTCTGTCCGCCATGGACAAGCTGTACTGCGAGTGTGGCGCCGTCATGACTTCGAAGCGCGGGGAAGAATCGATCAAGGACTCTTACCGCTGCCGTCGCCGGAAGGTGGTCGACCCGTCCGCACCTGGGCAGCACGAAGGCACGTGCAACGTCAGCATGGCGGCACTCGACAAGTTCGTTGCGGAACGCATCTTCAACAAGATCAGGCACGCCGAAGGCGACGAAGAGACGTTGGCGCTTCTGTGGGAAGCCGCCCGACGCTTCGGCAAGCTCACTGAGGCGCCTGAGAAGAGCGGCGAACGGGCGAACCTTGTTGCGGAGCGCGCCGACGCCCTGAACGCCCTTGAAGAGCTGTACGAAGACCGCGCGGCAGGCGCGTACGACGGACCCGTTGGCAGGAAGCACTTCCGGAAGCAACAGGCAGCGCTGACGCTCCGGCAGCAAGGGGCGGAAGAGCGGCTTGCCGAACTTGAAGCCGCCGAAGCCCCGAAGCTTCCCCTTGACCAATGGTTCCCCGAAGACGCCGACGCTGACCCGACCGGCCCTAAGTCGTGGTGGGGGCGCGCGTCAGTAGACGACAAGCGCGTGTTCGTCGGGCTCTTCGTAGACAAGATCGTTGTCACGAAGTCGACTACGGGCAGGGGGCAGGGAACGCCCATCGAGAAGCGCGCTTCGATCACGTGGGCGAAGCCGCCGACCGACGACGACGAAGACGACGCCCAGGACGGCACGGAAGACGTAGCGGCGTAG
Protein Sequence
MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDGGRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIPIVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQRELGGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWREIKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRILRDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQAWLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPGQHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERANLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAEAPKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPIEKRASITWAKPPTDDDEDDAQDGTEDVAA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| J23119(SpeI) promoter | — | — | 4953..4988 | Fwd | |
|
DNA Sequence
TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT
|
|||||
| lac promoter | — | — | 5240..5271 | Rev | |
|
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 5216..5233 | Fwd | |
|
DNA Sequence
TTGTTATCCGCTCACAA
|
|||||
| CAP binding site | — | — | 5285..5307 | Fwd | |
|
DNA Sequence
ATGAGTGAGCTAACTCACATTA
|
|||||
| phage phi-C31 attP | — | — | 8453..8553 | Fwd | |
|
DNA Sequence
CCCAGGTCAGAAGCGGTTTTCGGGAGTAGTGCCCCAACTGGGGTAACCTTTGAGTTCTCTCAGTTGGGGGCGTAGGGTCGCCGACATGACACAAGGGGTT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| ori | — | — | 5594..6183 | Rev | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lambda t0 terminator | — | — | 5100..5135 | Rev | |
|
DNA Sequence
TTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAT
|
|||||