| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| dCas9 | — | catalytically dead mutant of the Cas9 e… | 665..4772 | Fwd | |
|
DNA Sequence
ATGGACAAGAAGTACTCCATCGGCCTGGCGATCGGCACCAACTCCGTCGGCTGGGCCGTCATCACGGACGAGTACAAGGTCCCGTCGAAGAAGTTCAAGGTGCTGGGCAACACGGACCGCCACAGCATCAAGAAGAACCTCATCGGCGCCCTCCTGTTCGACAGCGGCGAGACGGCCGAGGCGACCCGCCTGAAGCGCACGGCGCGGCGGCGGTACACGCGCCGCAAGAACCGCATCTGCTACCTCCAGGAGATCTTCTCCAACGAGATGGCGAAGGTCGACGACTCGTTCTTCCACCGGCTGGAGGAGTCCTTCCTGGTCGAGGAGGACAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCGACCATCTACCACCTCCGGAAGAAGCTGGTGGACTCGACCGACAAGGCCGACCTGCGCCTGATCTACCTGGCCCTCGCCCACATGATCAAGTTCCGCGGCCACTTCCTCATCGAGGGCGACCTGAACCCGGACAACTCGGACGTCGACAAGCTGTTCATCCAGCTCGTGCAGACCTACAACCAGCTCTTCGAGGAGAACCCGATCAACGCCTCCGGCGTGGACGCGAAGGCCATCCTGTCGGCCCGCCTCTCGAAGTCCCGGCGGCTCGAGAACCTGATCGCCCAGCTGCCGGGCGAGAAGAAGAACGGCCTCTTCGGCAACCTGATCGCGCTCTCCCTGGGCCTGACGCCGAACTTCAAGTCCAACTTCGACCTCGCCGAGGACGCCAAGCTCCAGCTGTCCAAGGACACGTACGACGACGACCTGGACAACCTCCTGGCGCAGATCGGCGACCAGTACGCGGACCTGTTCCTCGCGGCGAAGAACCTCTCCGACGCGATCCTGCTCAGCGACATCCTCCGCGTCAACACCGAGATCACCAAGGCGCCCCTGTCCGCGAGCATGATCAAGCGCTACGACGAGCACCACCAGGACCTCACCCTGCTGAAGGCCCTGGTCCGCCAGCAGCTCCCGGAGAAGTACAAGGAGATCTTCTTCGACCAGTCCAAGAACGGCTACGCGGGCTACATCGACGGCGGCGCGTCCCAGGAGGAGTTCTACAAGTTCATCAAGCCCATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTGGTCAAGCTGAACCGGGAGGACCTCCTGCGCAAGCAGCGCACGTTCGACAACGGCTCCATCCCCCACCAGATCCACCTGGGCGAGCTGCACGCCATCCTCCGGCGCCAGGAGGACTTCTACCCGTTCCTCAAGGACAACCGCGAGAAGATCGAGAAGATCCTCACGTTCCGGATCCCGTACTACGTCGGCCCCCTCGCCCGCGGCAACTCCCGCTTCGCGTGGATGACGCGCAAGTCGGAGGAGACGATCACCCCGTGGAACTTCGAGGAGGTGGTGGACAAGGGCGCCTCCGCGCAGTCGTTCATCGAGCGGATGACCAACTTCGACAAGAACCTGCCGAACGAGAAGGTGCTGCCCAAGCACAGCCTCCTGTACGAGTACTTCACGGTCTACAACGAGCTCACCAAGGTGAAGTACGTGACGGAGGGCATGCGGAAGCCGGCGTTCCTGTCCGGCGAGCAGAAGAAGGCCATCGTGGACCTGCTGTTCAAGACGAACCGGAAGGTCACCGTCAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACTCGGTCGAGATCAGCGGCGTGGAGGACCGCTTCAACGCGTCGCTGGGCACGTACCACGACCTCCTGAAGATCATCAAGGACAAGGACTTCCTCGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTGCTGACCCTGACGCTCTTCGAGGACCGGGAGATGATCGAGGAGCGCCTCAAGACCTACGCGCACCTGTTCGACGACAAGGTCATGAAGCAGCTCAAGCGGCGGCGGTACACCGGCTGGGGCCGGCTCTCCCGCAAGCTCATCAACGGCATCCGGGACAAGCAGTCGGGCAAGACCATCCTCGACTTCCTGAAGTCCGACGGCTTCGCCAACCGCAACTTCATGCAGCTGATCCACGACGACAGCCTGACGTTCAAGGAGGACATCCAGAAGGCGCAGGTGTCGGGCCAGGGCGACTCCCTGCACGAGCACATCGCGAACCTGGCCGGCAGCCCGGCCATCAAGAAGGGCATCCTGCAGACGGTCAAGGTGGTCGACGAGCTGGTGAAGGTGATGGGCCGCCACAAGCCCGAGAACATCGTCATCGAGATGGCCCGGGAGAACCAGACCACGCAGAAGGGCCAGAAGAACAGCCGCGAGCGCATGAAGCGGATCGAGGAGGGCATCAAGGAGCTCGGCTCGCAGATCCTCAAGGAGCACCCCGTCGAGAACACCCAGCTCCAGAACGAGAAGCTCTACCTCTACTACCTGCAGAACGGCCGGGACATGTACGTGGACCAGGAGCTCGACATCAACCGGCTGTCGGACTACGACGTGGACGCCATCGTCCCGCAGTCGTTCCTGAAGGACGACAGCATCGACAACAAGGTCCTCACCCGCTCGGACAAGAACCGGGGCAAGTCCGACAACGTCCCGTCGGAGGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAGCTCCTCAACGCCAAGCTCATCACGCAGCGCAAGTTCGACAACCTGACGAAGGCGGAGCGGGGCGGCCTCAGCGAGCTCGACAAGGCGGGCTTCATCAAGCGGCAGCTGGTCGAGACGCGGCAGATCACGAAGCACGTGGCCCAGATCCTGGACTCGCGCATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGCGAGGTCAAGGTCATCACCCTCAAGTCCAAGCTGGTGAGCGACTTCCGCAAGGACTTCCAGTTCTACAAGGTGCGGGAGATCAACAACTACCACCACGCCCACGACGCGTACCTGAACGCGGTGGTGGGCACGGCGCTGATCAAGAAGTACCCGAAGCTCGAGAGCGAGTTCGTCTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCGAAGAGCGAGCAGGAGATCGGCAAGGCCACGGCGAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCGAACGGCGAGATCCGCAAGCGCCCGCTCATCGAGACCAACGGCGAGACGGGCGAGATCGTGTGGGACAAGGGCCGCGACTTCGCGACCGTCCGGAAGGTGCTGTCCATGCCCCAGGTCAACATCGTCAAGAAGACGGAGGTCCAGACCGGCGGCTTCAGCAAGGAGTCCATCCTGCCCAAGCGGAACAGCGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCCAAGAAGTACGGCGGCTTCGACTCCCCCACCGTCGCCTACTCGGTCCTCGTGGTCGCCAAGGTGGAGAAGGGCAAGAGCAAGAAGCTGAAGTCCGTGAAGGAGCTGCTGGGCATCACCATCATGGAGCGGTCGTCGTTCGAGAAGAACCCCATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTGAAGAAGGACCTCATCATCAAGCTCCCCAAGTACTCCCTGTTCGAGCTGGAGAACGGCCGGAAGCGCATGCTCGCCAGCGCGGGCGAGCTGCAGAAGGGCAACGAGCTGGCGCTGCCGAGCAAGTACGTCAACTTCCTGTACCTCGCCTCGCACTACGAGAAGCTGAAGGGCTCGCCGGAGGACAACGAGCAGAAGCAGCTGTTCGTGGAGCAGCACAAGCACTACCTCGACGAGATCATCGAGCAGATCTCCGAGTTCTCCAAGCGCGTCATCCTGGCCGACGCGAACCTGGACAAGGTCCTGTCCGCGTACAACAAGCACCGCGACAAGCCGATCCGGGAGCAGGCCGAGAACATCATCCACCTGTTCACGCTGACCAACCTCGGCGCCCCCGCGGCCTTCAAGTACTTCGACACCACGATCGACCGGAAGCGGTACACGAGCACGAAGGAGGTGCTCGACGCCACCCTGATCCACCAGAGCATCACGGGCCTGTACGAGACCCGCATCGACCTCTCCCAGCTGGGCGGCGACTGA
Protein Sequence
MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
|
|||||
| ApmR | aac(3)IV | aminoglycoside 3-N-acetyltransferase ty… | 6144..6945 | Fwd | |
|
DNA Sequence
ATGTCATCAGCGGTGGAGTGCAATGTCGTGCAATACGAATGGCGAAAAGCCGAGCTCATCGGTCAGCTTCTCAACCTTGGGGTTACCCCCGGCGGTGTGCTGCTGGTCCACAGCTCCTTCCGTAGCGTCCGGCCCCTCGAAGATGGGCCACTTGGACTGATCGAGGCCCTGCGTGCTGCGCTGGGTCCGGGAGGGACGCTCGTCATGCCCTCGTGGTCAGGTCTGGACGACGAGCCGTTCGATCCTGCCACGTCGCCCGTTACACCGGACCTTGGAGTTGTCTCTGACACATTCTGGCGCCTGCCAAATGTAAAGCGCAGCGCCCATCCATTTGCCTTTGCGGCAGCGGGGCCACAGGCAGAGCAGATCATCTCTGATCCATTGCCCCTGCCACCTCACTCGCCTGCAAGCCCGGTCGCCCGTGTCCATGAACTCGATGGGCAGGTACTTCTCCTCGGCGTGGGACACGATGCCAACACGACGCTGCATCTTGCCGAGTTGATGGCAAAGGTTCCCTATGGGGTGCCGAGACACTGCACCATTCTTCAGGATGGCAAGTTGGTACGCGTCGATTATCTCGAGAATGACCACTGCTGTGAGCGCTTTGCCTTGGCGGACAGGTGGCTCAAGGAGAAGAGCCTTCAGAAGGAAGGTCCAGTCGGTCATGCCTTTGCTCGGTTGATCCGCTCCCGCGACATTGTGGCGACAGCCCTGGGTCAACTGGGCCGAGATCCGTTGATCTTCCTGCATCCGCCAGAGGCGGGATGCGAAGAATGCGATGCCGCTCGCCAGTCGATTGGC
Protein Sequence
MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRPLEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNVKRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLHLAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGHAFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG
|
|||||
| traJ | traJ | oriT-recognizing protein | 7378..7750 | Rev | |
|
DNA Sequence
ATGGCTGATGAAACCAAGCCAACCAGGAAGGGCAGCCCACCTATCAAGGTGTACTGCCTTCCAGACGAACGAAGAGCGATTGAGGAAAAGGCGGCGGCGGCCGGCATGAGCCTGTCGGCCTACCTGCTGGCCGTCGGCCAGGGCTACAAAATCACGGGCGTCGTGGACTATGAGCACGTCCGCGAGCTGGCCCGCATCAATGGCGACCTGGGCCGCCTGGGCGGCCTGCTGAAACTCTGGCTCACCGACGACCCGCGCACGGCGCGGTTCGGTGATGCCACGATCCTCGCCCTGCTGGCGAAGATCGAAGAGAAGCAGGACGAGCTTGGCAAGGTCATGATGGGCGTGGTCCGCCCGAGGGCAGAGCCATGA
Protein Sequence
MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAVGQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEEKQDELGKVMMGVVRPRAEP
|
|||||
| Factor Xa site | — | Factor Xa recognition and cleavage site | 8129..8141 | Rev | |
|
DNA Sequence
ATAGAGGGGCGT
Protein Sequence
IEGR
|
|||||
| phage phi-C31 integrase | — | integrase from phage phi-C31 | 8320..10138 | Fwd | |
|
DNA Sequence
GTGGACACGTACGCGGGTGCTTACGACCGTCAGTCGCGCGAGCGCGAAAATTCGAGCGCAGCAAGCCCAGCGACACAGCGTAGCGCCAACGAAGACAAGGCGGCCGACCTTCAGCGCGAAGTCGAGCGCGACGGGGGCCGGTTCAGGTTCGTCGGGCATTTCAGCGAAGCGCCGGGCACGTCGGCGTTCGGGACGGCGGAGCGCCCGGAGTTCGAACGCATCCTGAACGAATGCCGCGCCGGGCGGCTCAACATGATCATTGTCTATGACGTGTCGCGCTTCTCGCGCCTGAAGGTCATGGACGCGATTCCGATTGTCTCGGAATTGCTCGCCCTGGGCGTGACGATTGTTTCCACTCAGGAAGGCGTCTTCCGGCAGGGAAACGTCATGGACCTGATTCACCTGATTATGCGGCTCGACGCGTCGCACAAAGAATCTTCGCTGAAGTCGGCGAAGATTCTCGACACGAAGAACCTTCAGCGCGAATTGGGCGGGTACGTCGGCGGGAAGGCGCCTTACGGCTTCGAGCTTGTTTCGGAGACGAAGGAGATCACGCGCAACGGCCGAATGGTCAATGTCGTCATCAACAAGCTTGCGCACTCGACCACTCCCCTTACCGGACCCTTCGAGTTCGAGCCCGACGTAATCCGGTGGTGGTGGCGTGAGATCAAGACGCACAAACACCTTCCCTTCAAGCCGGGCAGTCAAGCCGCCATTCACCCGGGCAGCATCACGGGGCTTTGTAAGCGCATGGACGCTGACGCCGTGCCGACCCGGGGCGAGACGATTGGGAAGAAGACCGCTTCAAGCGCCTGGGACCCGGCAACCGTTATGCGAATCCTTCGGGACCCGCGTATTGCGGGCTTCGCCGCTGAGGTGATCTACAAGAAGAAGCCGGACGGCACGCCGACCACGAAGATTGAGGGTTACCGCATTCAGCGCGACCCGATCACGCTCCGGCCGGTCGAGCTTGATTGCGGACCGATCATCGAGCCCGCTGAGTGGTATGAGCTTCAGGCGTGGTTGGACGGCAGGGGGCGCGGCAAGGGGCTTTCCCGGGGGCAAGCCATTCTGTCCGCCATGGACAAGCTGTACTGCGAGTGTGGCGCCGTCATGACTTCGAAGCGCGGGGAAGAATCGATCAAGGACTCTTACCGCTGCCGTCGCCGGAAGGTGGTCGACCCGTCCGCACCTGGGCAGCACGAAGGCACGTGCAACGTCAGCATGGCGGCACTCGACAAGTTCGTTGCGGAACGCATCTTCAACAAGATCAGGCACGCCGAAGGCGACGAAGAGACGTTGGCGCTTCTGTGGGAAGCCGCCCGACGCTTCGGCAAGCTCACTGAGGCGCCTGAGAAGAGCGGCGAACGGGCGAACCTTGTTGCGGAGCGCGCCGACGCCCTGAACGCCCTTGAAGAGCTGTACGAAGACCGCGCGGCAGGCGCGTACGACGGACCCGTTGGCAGGAAGCACTTCCGGAAGCAACAGGCAGCGCTGACGCTCCGGCAGCAAGGGGCGGAAGAGCGGCTTGCCGAACTTGAAGCCGCCGAAGCCCCGAAGCTTCCCCTTGACCAATGGTTCCCCGAAGACGCCGACGCTGACCCGACCGGCCCTAAGTCGTGGTGGGGGCGCGCGTCAGTAGACGACAAGCGCGTGTTCGTCGGGCTCTTCGTAGACAAGATCGTTGTCACGAAGTCGACTACGGGCAGGGGGCAGGGAACGCCCATCGAGAAGCGCGCTTCGATCACGTGGGCGAAGCCGCCGACCGACGACGACGAAGACGACGCCCAGGACGGCACGGAAGACGTAGCGGCGTAG
Protein Sequence
MDTYAGAYDRQSRERENSSAASPATQRSANEDKAADLQREVERDGGRFRFVGHFSEAPGTSAFGTAERPEFERILNECRAGRLNMIIVYDVSRFSRLKVMDAIPIVSELLALGVTIVSTQEGVFRQGNVMDLIHLIMRLDASHKESSLKSAKILDTKNLQRELGGYVGGKAPYGFELVSETKEITRNGRMVNVVINKLAHSTTPLTGPFEFEPDVIRWWWREIKTHKHLPFKPGSQAAIHPGSITGLCKRMDADAVPTRGETIGKKTASSAWDPATVMRILRDPRIAGFAAEVIYKKKPDGTPTTKIEGYRIQRDPITLRPVELDCGPIIEPAEWYELQAWLDGRGRGKGLSRGQAILSAMDKLYCECGAVMTSKRGEESIKDSYRCRRRKVVDPSAPGQHEGTCNVSMAALDKFVAERIFNKIRHAEGDEETLALLWEAARRFGKLTEAPEKSGERANLVAERADALNALEELYEDRAAGAYDGPVGRKHFRKQQAALTLRQQGAEERLAELEAAEAPKLPLDQWFPEDADADPTGPKSWWGRASVDDKRVFVGLFVDKIVVTKSTTGRGQGTPIEKRASITWAKPPTDDDEDDAQDGTEDVAA
|
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| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac promoter | — | — | 4998..5029 | Rev | |
|
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| lac operator | — | — | 4974..4991 | Fwd | |
|
DNA Sequence
TTGTTATCCGCTCACAA
|
|||||
| CAP binding site | — | — | 5043..5065 | Fwd | |
|
DNA Sequence
ATGAGTGAGCTAACTCACATTA
|
|||||
| phage phi-C31 attP | — | — | 8211..8311 | Fwd | |
|
DNA Sequence
CCCAGGTCAGAAGCGGTTTTCGGGAGTAGTGCCCCAACTGGGGTAACCTTTGAGTTCTCTCAGTTGGGGGCGTAGGGTCGCCGACATGACACAAGGGGTT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| ori | — | — | 5352..5941 | Rev | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
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