pYES2.1-TRP1URA3

11008 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Francisco Malagon
Insert
TRP1URA3 fusion
Tags
V5 and his6
Mutation
ORF-TRP1 fused to ORF-URA without stop codon
Available Since
Dec. 16, 2005
Availability
Available

Sequence Information

Length
715 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
6xHis — 6xHis affinity tag 8..26 Rev
DNA Sequence
CATCATCACCATCACCAT
Protein Sequence
HHHHHH
V5 tag — epitope tag from simian virus 5 35..77 Rev
DNA Sequence
GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACG
Protein Sequence
GKPIPNPLLGLDST