pYES2.1-TRP1

11007 Saccharomyces cerevisiae Bacterial Expression

Plasmid Information

Depositor
Francisco Malagon
Insert
TRP1 (TRP1 Budding Yeast)
Tags
V5 and his6
Mutation
ORF-TRP1 without stop codon
Available Since
Dec. 16, 2005
Availability
Available

Sequence Information

Length
812 bp
Topology
linear
Molecule Type
genomic DNA
View on AddGene.org

Features

CDS 2
Label Gene Product Position Strand
V5 tag — epitope tag from simian virus 5 695..737 Fwd
DNA Sequence
GGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACG
Protein Sequence
GKPIPNPLLGLDST
6xHis — 6xHis affinity tag 746..764 Fwd
DNA Sequence
CATCATCACCATCACCAT
Protein Sequence
HHHHHH