pFC902

106904 Synthetic Bacterial Expression

Plasmid Information

Purpose
The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1
Depositor
Uffe Mortensen
Article
Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108
Tags
SV40 NLS
Available Since
May 11, 2018
Availability
Available

Sequence Information

Length
16,689 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

CDS 3
Label Gene Product Position Strand
AmpR bla beta-lactamase 0..16689 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGTAGCGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCGATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
Cas9 — Cas9 (Csn1) endonuclease from the Strep… 8945..13049 Fwd
DNA Sequence
ATGGACAAGAAGTATAGCATCGGGCTGGACATTGGAACGAACTCGGTTGGTTGGGCTGTGATTACGGACGAATACAAGGTGCCATCCAAGAAGTTTAAGGTCCTGGGAAACACCGACCGTCACTCAATCAAGAAGAATCTCATTGGAGCCCTGCTCTTCGATAGTGGGGAGACCGCCGAAGCTACTCGACTGAAGCGAACGGCTCGCCGGCGTTATACACGACGCAAGAATCGCATCTGCTACCTCCAGGAGATTTTCAGCAACGAAATGGCTAAGGTTGATGACTCATTCTTTCATCGACTCGAAGAAAGTTTCTTGGTCGAGGAGGATAAGAAGCACGAGCGCCATCCGATCTTTGGTAACATTGTGGATGAGGTTGCCTATCACGAAAAGTACCCAACTATCTATCATCTTCGTAAGAAGCTGGTCGATAGCACGGACAAGGCTGATTTGCGACTTATCTACCTGGCACTCGCGCACATGATTAAGTTCCGCGGCCATTTTCTTATCGAGGGTGACCTGAACCCCGATAATTCTGACGTTGATAAGCTCTTCATCCAGTTGGTCCAAACCTACAATCAGCTGTTTGAGGAAAACCCTATTAATGCATCTGGCGTGGACGCCAAGGCTATCCTTTCGGCGCGCCTGTCTAAGTCGCGGCGTTTGGAGAACCTTATCGCACAACTCCCCGGCGAAAAGAAGAACGGCCTCTTCGGTAATTTGATTGCGTTGTCACTTGGTCTGACTCCTAACTTCAAGAGTAATTTTGACCTGGCAGAGGATGCGAAGCTCCAGTTGTCTAAGGATACGTATGATGACGATCTCGACAACTTGCTTGCCCAAATCGGTGACCAGTACGCTGATCTTTTCCTGGCCGCTAAGAATCTCTCAGATGCAATCCTGCTCAGTGACATTTTGCGGGTCAACACCGAGATTACTAAGGCCCCCCTGTCAGCTAGTATGATCAAGCGGTATGATGAGCACCATCAGGACCTCACCTTGCTTAAGGCCCTCGTGCGTCAGCAATTGCCTGAGAAGTATAAGGAAATCTTCTTTGACCAATCCAAGAACGGATACGCAGGGTATATTGATGGCGGTGCGAGCCAGGAGGAATTCTACAAGTTTATCAAGCCGATTTTGGAGAAGATGGACGGCACTGAGGAACTGCTCGTCAAGCTGAATCGCGAAGATTTGCTTCGTAAGCAACGAACGTTCGACAACGGCTCCATCCCGCACCAGATTCATCTGGGCGAGCTCCACGCCATCCTTCGACGCCAGGAAGATTTCTACCCATTTCTGAAGGACAACCGTGAGAAGATCGAAAAGATTCTTACATTCCGAATCCCCTACTATGTGGGACCTTTGGCCCGTGGGAATTCCCGATTTGCTTGGATGACCCGAAAGAGCGAGGAAACCATCACTCCGTGGAACTTCGAGGAAGTCGTGGACAAGGGTGCATCCGCGCAGAGCTTCATTGAGCGGATGACCAATTTTGATAAGAACCTTCCGAATGAAAAGGTCCTGCCAAAGCATTCGCTGCTCTACGAGTATTTCACCGTGTATAACGAACTGACTAAGGTCAAGTACGTGACGGAGGGAATGCGGAAGCCAGCCTTCCTCTCAGGGGAACAAAAGAAGGCTATCGTCGATTTGCTTTTTAAGACCAATCGTAAAGTGACTGTTAAGCAGCTGAAGGAGGATTATTTCAAGAAGATTGAATGTTTCGACTCCGTCGAGATCAGCGGCGTGGAAGATCGCTTTAACGCTTCCCTCGGTACCTACCACGACCTGCTCAAGATCATTAAGGACAAGGATTTCCTCGATAACGAGGAAAATGAGGACATCTTGGAAGATATTGTCCTCACGTTGACACTTTTTGAGGACCGCGAAATGATCGAGGAACGGCTCAAGACATATGCCCATTTGTTCGACGATAAGGTGATGAAGCAGCTGAAGCGGCGTCGATACACCGGATGGGGTCGCCTTAGCCGGAAGCTGATCAACGGCATTCGAGATAAGCAATCTGGTAAGACTATCTTGGATTTCCTTAAGTCGGACGGCTTCGCCAACCGCAATTTTATGCAGCTTATTCACGACGATTCCCTGACGTTCAAGGAGGACATCCAGAAGGCACAAGTCTCAGGACAAGGGGATTCCCTGCACGAGCATATCGCCAACCTGGCTGGATCCCCGGCGATCAAGAAGGGGATTCTTCAGACCGTCAAGGTTGTCGACGAGCTGGTCAAGGTGATGGGCCGTCATAAGCCAGAAAACATCGTGATTGAGATGGCCCGAGAAAATCAGACCACTCAAAAGGGTCAGAAGAACAGCCGCGAGCGGATGAAGCGGATCGAGGAAGGCATTAAGGAACTTGGTTCTCAGATCCTGAAGGAGCACCCTGTTGAAAACACACAGCTCCAAAATGAGAAGCTGTATCTCTACTATTTGCAAAATGGACGCGACATGTACGTCGATCAGGAGCTCGACATTAACCGGTTGTCGGACTACGATGTTGACCATATCGTCCCGCAATCCTTCCTTAAGGACGATAGCATTGATAACAAGGTGCTGACTCGCTCAGATAAGAACCGGGGCAAGTCCGACAATGTTCCAAGCGAGGAAGTGGTTAAGAAGATGAAGAACTACTGGCGCCAATTGCTTAATGCCAAGCTCATCACACAGCGCAAGTTTGACAACTTGACCAAGGCCGAGCGGGGAGGGCTGAGTGAACTCGATAAGGCTGGCTTCATCAAGCGTCAACTCGTGGAGACGCGACAGATCACAAAGCACGTTGCTCAGATTCTGGACTCCCGGATGAACACAAAGTACGACGAGAATGATAAGCTCATCCGTGAAGTTAAGGTCATTACCCTCAAGTCTAAGTTGGTGTCGGATTTCCGCAAGGACTTCCAATTTTATAAGGTTCGGGAGATCAACAATTATCACCATGCACATGATGCGTACCTCAACGCAGTCGTGGGAACTGCGCTCATCAAGAAGTATCCCAAGTTGGAGTCCGAATTCGTCTACGGGGATTATAAGGTTTACGACGTCCGCAAGATGATCGCCAAGAGTGAGCAGGAAATTGGCAAGGCCACGGCTAAGTATTTCTTTTACTCCAACATCATGAATTTCTTTAAGACGGAGATCACACTCGCCAATGGAGAAATCCGTAAGCGACCTTTGATTGAGACCAACGGCGAGACTGGTGAAATCGTTTGGGATAAGGGGCGCGACTTCGCTACCGTGCGGAAGGTTCTGAGCATGCCGCAAGTCAATATCGTCAAGAAAACCGAGGTGCAGACAGGCGGTTTCTCTAAGGAATCGATTCTTCCAAAGCGTAACTCTGACAAGCTGATCGCTCGAAAGAAGGATTGGGACCCCAAGAAGTATGGAGGGTTCGATTCTCCTACAGTGGCATACTCGGTTCTCGTTGTCGCGAAGGTTGAGAAGGGAAAGTCTAAGAAGCTGAAGTCGGTCAAGGAACTGCTCGGGATCACCATTATGGAGCGCTCCAGCTTCGAAAAGAATCCCATCGACTTTCTCGAGGCCAAGGGCTATAAGGAAGTCAAGAAGGATCTTATCATTAAGCTGCCTAAGTACTCTTTGTTCGAGCTTGAAAACGGTCGAAAGCGAATGCTCGCATCGGCAGGAGAGTTGCAGAAGGGGAATGAATTGGCACTTCCCTCAAAGTACGTGAACTTCCTGTATCTCGCGTCCCACTACGAGAAGCTGAAGGGTAGCCCTGAGGACAACGAACAGAAGCAACTTTTTGTTGAGCAACACAAGCATTATCTGGATGAGATCATTGAACAGATTTCAGAGTTCAGTAAGCGCGTCATCCTCGCCGATGCTAATCTCGACAAGGTGTTGTCGGCCTACAACAAGCACCGTGACAAGCCGATCCGAGAGCAGGCTGAAAATATCATTCATCTGTTCACCCTCACTAACTTGGGAGCACCAGCAGCGTTCAAGTATTTTGATACGACAATCGACCGTAAGCGATACACGTCCACAAAGGAGGTGCTTGATGCGACCCTGATTCATCAATCCATCACTGGGCTCTATGAAACCCGTATCGACCTTAGTCAACTGGGGGGCGAC
Protein Sequence
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
SV40 NLS — nuclear localization signal of SV40 (si… 13052..13073 Fwd
DNA Sequence
CCCAAGAAGAAGCGCAAGGTC
Protein Sequence
PKKKRKV
promoter 1
Label Gene Product Position Strand
AmpR promoter bla — 859..951 Rev
DNA Sequence
TTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAAT
protein_bind 2
Label Gene Product Position Strand
FRT (minimal) — — 1243..1277 Fwd
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
FRT (minimal) — — 15188..15222 Rev
DNA Sequence
GAAGTTCCTATTCTCTAGAAAGTATAGGAACTTC
rep_origin 2
Label Gene Product Position Strand
AMA1 — — 1589..6836 Fwd
DNA Sequence
AGCTTATTTTTTGTATACTGTTTTGTGATAGCACGAAGTTTTTCCACGGTATCTTGTTAAAAATATATATTTGTGGCGGGCTTACCTACATCAAATTAATAAGAGACTAATTATAAACTAAACACACAAGCAAGCTACTTTAGGGTAAAAGTTTATAAATGCTTTTGACGTATAAACGTTGCTTGTATTTATTATTACAATTAAAGGTGGATAGAAAACCTAGAGACTAGTTAGAAACTAATCTCAGGTTTGCGTTAAACTAAATCAGAGCCCGAGAGGTTAACAGAACCTAGAAGGGGACTAGATATCCGGGTAGGGAAACAAAAAAAAAAACAAGACAGCCACATATTAGGGAGACTAGTTAGAAGCTAGTTCCAGGACTAGGAAAATAAAAGACAATGATACCACAGTCTAGTTGACAACTAGATAGATTCTAGATTGAGGCCAAAGTCTCTGAGATCCAGGTTAGTTGCAACTAATACTAGTTAGTATCTAGTCTCCTATAACTCTGAAGCTAGAATAACTTACTACTATTATCCTCACCACTGTTCAGCTGCGCAAACGGAGTGATTGCAAGGTGTTCAGAGACTAGTTATTGACTAGTCAGTGACTAGCAATAACTAACAAGGTATTAACCTACCATGTCTGCCATCACCCTGCACTTCCTCGGGCTCAGCAGCCTTTTCCTCCTCATTTTCATGCTCATTTTCCTTGTTTAAGACTGTGACTAGTCGAAGACTAGTCCAGAACCACAAAGGAGAAATGTCTTACCACTTTCTTCATTGCTTGTCTCTTTTGCATTATCCATGTCTGCAACTAGTTAGAGTCTAGTTAGTGACTAGTCCGACGAGGACTTGCTTGTCTCCGGATTGTTGGAGGAACTCTCCAGGGCCTCAAGATCCACAACAGAGCCTTCTAGAAGACTGGTCAATAACTAGTTGGTCTTTGTCTGAGTCTGACTTACGAGGTTGCATACTCGCTCCCTTTGCCTCGTCAATCGATGAGAAAAAGCGCCAAAACTCGCAATATGGCTTTGAACCACACGGTGCTGAGACTAGTTAGAATCTAGTCCCAAACTAGCTTGGATAGCTTACCTTTGCCCTTTGCGTTGCGACAGGTCTTGCAGGGTATGGTTCCTTTCTCACCAGCTGATTTAGCTGCCTTGCTACCCTCACGGCGGATCTGCATAAAGAGTGGCTAGAGGTTATAAATTAGCACTGATCCTAGGTACGGGGCTGAATGTAACTTGCCCTTCCTTTCTCATCGCGCGGCAAGACAGGCTTGCTCAAATTCCTACCAGTCACAGGGGTATGCACGGCGTACGGACCACTTGAACTAGTCACAGATTAGTTAGCAACTAGTCTGCATTGAATGGCTGTACTTACGGGCCCTCGCCATTGTCCTGATCATTTCCAGCTTCACCCTCGTTGCTGCAAAGTAGTTAGTGACTAGTCAAGGACTAGTTGAAATGGGAGAAGAAACTCACGAATTCTCGACACCCTTAGTATTGTGGTCCTTGGACTTGGTGCTGCTATATATTAGCTAATACACTAGTTAGACTCACGGAAACTTACGCAGCTCGCTTGCGCTTCTTGGTAGGAGTCGGGGTTGGGAGAACAGTGCCTTCAAACAAGCCTTCATACCATGCTACTTGACTAGTCAGGGACTAGTCACCAAGTAATCTAGATAGGACTTGCCTTTGGCCTCCATCAGTTCCTTCATAGTGGGAGGTCCATTGTGCAATGTAAACTCCATGCCGTGGGAGTTCTTGTCCTTCAAGTGCTTGACCAATATGTTTCTGTTGGCAGAGGGAACCTGTCAACTAGTTAATAACTAGTCAGAAACTAGTATAGCAGTAGACTCACTGTACGCTTGAGGCATCCCTTCACTCGGCAGTAGACTTCATATGGATGGATATCAGGCACGCCATTGTCGTCCTGTGGACTAGTCAGTAACTAGGCTTAAAGCTAGTCGGGTCGGCTTACTATCTTGAAATCCGGCAGCGTAAGCTCCCCGTCCTTAACTGCCTCGAGATAGTGACAGTACTCTGGGGACTTTCGGAGATCGTTATCGCGAATGCTCGGCATACTAATCGTTGACTAGTCTTGGACTAGTCCCGAGCAAAAAGGATTGGAGGAGGAGGAGGAAGGTGAGAGTGAGACAAAGAGCGAAATAAGAGCTTCAAAGGCTATCTCTAAGCAGTATGAAGGTTAAGTATCTAGTTCTTGACTAGATTTAAAAGAGATTTCGACTAGTTATGTACCTGGAGTTTGGATATAGGAATGTGTTGTGGTAACGAAATGTAAGGGGGAGGAAAGAAAAAGTCGGTCAAGAGGTAACTCTAAGTCGGCCATTCCTTTTTGGGAGGCGCTAACCATAAACGGCATGGTCGACTTAGAGTTAGCTCAGGGAATTTAGGGAGTTATCTGCGACCACCGAGGAACGGCGGAATGCCAAAGAATCCCGATGGAGCTCTAGCTGGCGGTTGACAACCCCACCTTTTGGCGTTTCTGCGGCGTTGCAGGCGGGACTGGATACTTCGTAGAACCAGAAAGGCAAGGCAGAACGCGCTCAGCAAGAGTGTTGGAAGTGATAGCATGATGTGCCTTGTTAACTAGGTCAAAATCTGCAGTATGCTTGATGTTATCCAAAGTGTGAGAGAGGAAGGTCCAAACATACACGATTGGGAGAGGGCCTAGGTATAAGAGTTTTTGAGTAGAACGCATGTGAGCCCAGCCATCTCGAGGAGATTAAACACGGGCCGGCATTTGATGGCTATGTTAGTACCCCAATGGAAACGGTGAGAGTCCAGTGGTCGCAGATAACTCCCTAAATTCCCTGAGCTAACTCTAAGTCGACCATGCCGTTTATGGTTAGCGCCTCCCAAAAAGGAATGGCCGACTTAGAGTTACCTCTTGACCGACTTTTTCTTTCCTCCCCCTTACATTTCGTTACCACAACACATTCCTATATCCAAACTCCAGGTACATAACTAGTCGAAATCTCTTTTAAATCTAGTCAAGAACTAGATACTTAACCTTCATACTGCTTAGAGATAGCCTTTGAAGCTCTTATTTCGCTCTTTGTCTCACTCTCACCTTCCTCCTCCTCCTCCAATCCTTTTTGCTCGGGACTAGTCCAAGACTAGTCAACGATTAGTATGCCGAGCATTCGCGATAACGATCTCCGAAAGTCCCCAGAGTACTGTCACTATCTCGAGGCAGTTAAGGACGGGGAGCTTACGCTGCCGGATTTCAAGATAGTAAGCCGACCCGACTAGCTTTAAGCCTAGTTACTGACTAGTCCACAGGACGACAATGGCGTGCCTGATATCCATCCATATGAAGTCTACTGCCGAGTGAAGGGATGCCTCAAGCGTACAGTGAGTCTACTGCTATACTAGTTTCTGACTAGTTATTAACTAGTTGACAGGTTCCCTCTGCCAACAGAAACATATTGGTCAAGCACTTGAAGGACAAGAACTCCCACGGCATGGAGTTTACATTGCACAATGGACCTCCCACTATGAAGGAACTGATGGAGGCCAAAGGCAAGTCCTATCTAGATTACTTGGTGACTAGTCCCTGACTAGTCAAGTAGCATGGTATGAAGGCTTGTTTGAAGGCACTGTTCTCCCAACCCCGACTCCTACCAAGAAGCGCAAGCGAGCTGCGTAAGTTTCTGTGAGTCTAACTAGTGTATTAGCTAATATATAGCAGCACCAAGTCCAAGGACCACAATACTAAGGGTGTCGAGAATTCGTGAGTTTCTTCTCCCATTTCAACTAGTCCTTGACTAGTCACTAACTACTTTGCAGCAACGAGGGTGAAGCTGGAAATGATCAGGACAATGGCGAGGGCCCGTAAGTACAGCCATTCAATGCAGACTAGTTGCTAACTAATCTGTGACTAGTTCAAGTGGTCCGTACGCCGTGCATACCCCTGTGACTGGTAGGAATTTGAGCAAGCCTGTCTTGCCGCGCGATGAGAAAGGAAGGGCAAGTTACATTCAGCCCCGTACCTAGGATCAGTGCTAATTTATAACCTCTAGCCACTCTTTATGCAGATCCGCCGTGAGGGTAGCAAGGCAGCTAAATCAGCTGGTGAGAAAGGAACCATACCCTGCAAGACCTGTCGCAACGCAAAGGGCAAAGGTAAGCTATCCAAGCTAGTTTGGGACTAGATTCTAACTAGTCTCAGCACCGTGTGGTTCAAAGCCATATTGCGAGTTTTGGCGCTTTTTCTCATCGATTGACGAGGCAAAGGGAGCGAGTATGCAACCTCGTAAGTCAGACTCAGACAAAGACCAACTAGTTATTGACCAGTCTTCTAGAAGGCTCTGTTGTGGATCTTGAGGCCCTGGAGAGTTCCTCCAACAATCCGGAGACAAGCAAGTCCTCGTCGGACTAGTCACTAACTAGACTCTAACTAGTTGCAGACATGGATAATGCAAAAGAGACAAGCAATGAAGAAAGTGGTAAGACATTTCTCCTTTGTGGTTCTGGACTAGTCTTCGACTAGTCACAGTCTTAAACAAGGAAAATGAGCATGAAAATGAGGAGGAAAAGGCTGCTGAGCCCGAGGAAGTGCAGGGTGATGGCAGACATGGTAGGTTAATACCTTGTTAGTTATTGCTAGTCACTGACTAGTCAATAACTAGTCTCTGAACACCTTGCAATCACTCCGTTTGCGCAGCTGAACAGTGGTGAGGATAATAGTAGTAAGTTATTCTAGCTTCAGAGTTATAGGAGACTAGATACTAACTAGTATTAGTTGCAACTAACCTGGATCTCAGAGACTTTGGCCTCAATCTAGAATCTATCTAGTTGTCAACTAGACTGTGGTATCATTGTCTTTTATTTTCCTAGTCCTGGAACTAGCTTCTAACTAGTCTCCCTAATATGTGGCTGTCTTGTTTTTTTTTTTGTTTCCCTACCCGGATATCTAGTCCCCTTCTAGGTTCTGTTAACCTCTCGGGCTCTGATTTAGTTTAACGCAAACCTGAGATTAGTTTCTAACTAGTCTCTAGGTTTTCTATCCACCTTTAATTGTAATAATAAATACAAGCAACGTTTATACGTCAAAAGCATTTATAAACTTTTACCCTAAAGTAGCTTGCTTGTGTGTTTAGTTTATAATTAGTCTCTTATTAATTTGATGTAGGTAAGCCCGCCACAAATATATATTTTTAACAAGATACCGTGGAAAAACTTCGTGCTATCACAAAACAGTATACAAAAAATAAGCT
ori — — 15977..16566 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA