pAP2050-2

105243 Homo sapiens Bacterial Expression

Plasmid Information

Purpose
mammalian GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker
Depositor
Geraldine Seydoux
Article
Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p
Insert
GFP11 with extra-sequence (GFP11 with linker::TEV::3xFlag::Ollas::Myc::V5::HAtag::other linker)
Available Since
Feb. 22, 2018
Availability
Available

Sequence Information

Length
3,026 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 8
Label Gene Product Position Strand
GFP11 11th beta-strand of superfolder GFP 375..423 Fwd
DNA Sequence
CGTGACCACATGGTCCTTCATGAGTATGTAAATGCTGCTGGGATTACA
Protein Sequence
RDHMVLHEYVNAAGIT
TEV site tobacco etch virus (TEV) protease recog… 432..453 Fwd
DNA Sequence
GAGAACCTTTATTTTCAGGGA
Protein Sequence
ENLYFQG
3xFLAG three tandem FLAG(R) epitope tags, foll… 453..519 Fwd
DNA Sequence
GACTATAAGGACCATGACGGGGATTATAAAGATCACGACATTGATTACAAGGACGACGACGATAAG
Protein Sequence
DYKDHDGDYKDHDIDYKDDDDK
OLLAS tag Escherichia coli OmpF Linker and mouse … 519..561 Fwd
DNA Sequence
TCCGGCTTCGCTAACGAATTGGGGCCAAGGCTCATGGGCAAG
Protein Sequence
SGFANELGPRLMGK
Myc Myc (human c-Myc proto-oncogene) epitop… 561..591 Fwd
DNA Sequence
GAACAGAAATTGATCTCTGAGGAGGACCTG
Protein Sequence
EQKLISEEDL
V5 tag epitope tag from simian virus 5 591..633 Fwd
DNA Sequence
GGTAAACCTATCCCAAATCCCCTGTTGGGTTTGGACTCAACT
Protein Sequence
GKPIPNPLLGLDST
HA HA (human influenza hemagglutinin) epit… 633..660 Fwd
DNA Sequence
TACCCCTACGACGTTCCTGACTATGCT
Protein Sequence
YPYDVPDYA
AmpR bla beta-lactamase 1919..2780 Rev
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 2
Label Gene Product Position Strand
lac promoter 806..837 Rev
DNA Sequence
TTTACACTTTATGCTTCCGGCTCGTATGTTG
AmpR promoter bla 2780..2885 Rev
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 2
Label Gene Product Position Strand
lac operator 782..799 Fwd
DNA Sequence
TTGTTATCCGCTCACAA
CAP binding site 851..873 Fwd
DNA Sequence
ATGAGTGAGCTAACTCACATTA
rep_origin 1
Label Gene Product Position Strand
ori 1160..1749 Rev
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA