pSBY2_ligD(FnCpf1)

104623 Synthetic Bacterial Expression

Plasmid Information

Purpose
CRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligD
Depositor
Sheng Yang
Article
Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d
Insert
crRNA to ligD
Promoter
Pj23119
Available Since
June 25, 2018
Availability
Available

Sequence Information

Length
6,043 bp
Topology
circular
Molecule Type
other DNA
View on AddGene.org

Features

rep_origin 1
Label Gene Product Position Strand
ori — — 4817..5406 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 3
Label Gene Product Position Strand
rrnB T2 terminator — — 5666..5694 Fwd
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT
rrnB T1 terminator Escherichia coli rrnB — 5813..5899 Fwd
DNA Sequence
AAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAT
rrnB T2 terminator — — 5990..6018 Fwd
DNA Sequence
AGAAGGCCATCCTGACGGATGGCCTTTT