PB-TRE3G-SOX17 — PlasmidDB

Plasmid Information

Purpose

PiggyBac vector encoding doxycycline-inducible human SOX17

Depositor

David Vereide

Article

Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

Insert

SRY-box 17 (SOX17 Human)

Promoter

TRE3G

Available Since

Jan. 10, 2018

Availability

Available

Length

6,425 bp

Topology

circular

Molecule Type

other DNA

CDS 3

Label	Gene	Product	Position	Strand
HQ tag	—	HQHQHQ metal affinity tag	2815..2833	Fwd
DNA Sequence CACCAGCACCAGCACCAG Protein Sequence HQHQHQ
HQ tag	—	HQHQHQ metal affinity tag	2821..2839	Fwd
DNA Sequence CACCAGCACCAGCACCAG Protein Sequence HQHQHQ
AmpR	bla	beta-lactamase	5366..6227	Rev
DNA Sequence ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA Protein Sequence MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW

polyA_signal 1

Label	Gene	Product	Position	Strand
SV40 poly(A) signal	—	—	3202..3324	Fwd
DNA Sequence TAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT

promoter 3

Label	Gene	Product	Position	Strand
TRE3G promoter	—	—	1429..1808	Fwd
DNA Sequence GAGTTTACTCCCTATCAGTGATAGAGAACGTATGAAGAGTTTACTCCCTATCAGTGATAGAGAACGTATGCAGACTTTACTCCCTATCAGTGATAGAGAACGTATAAGGAGTTTACTCCCTATCAGTGATAGAGAACGTATGACCAGTTTACTCCCTATCAGTGATAGAGAACGTATCTACAGTTTACTCCCTATCAGTGATAGAGAACGTATATCCAGTTTACTCCCTATCAGTGATAGAGAACGTATAAGCTTTAGGCGTGTACGGTGGGCGCCTATAAAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTGGAGCAATTCCACAACACTTTTGTCTTATACCAACTTTCCGTACCACTTCCTACCCTCGTAAA
lac promoter	—	—	4253..4284	Rev
DNA Sequence TTTACACTTTATGCTTCCGGCTCGTATGTTG
AmpR promoter	bla	—	6227..6332	Rev
DNA Sequence CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT

protein_bind 12

Label	Gene	Product	Position	Strand
tet operator	tetO	—	1437..1456	Fwd
DNA Sequence TCCCTATCAGTGATAGAGA
tet operator	tetO	—	1473..1492	Fwd
DNA Sequence TCCCTATCAGTGATAGAGA
tet operator	tetO	—	1509..1528	Fwd
DNA Sequence TCCCTATCAGTGATAGAGA
tet operator	tetO	—	1545..1564	Fwd
DNA Sequence TCCCTATCAGTGATAGAGA
tet operator	tetO	—	1581..1600	Fwd
DNA Sequence TCCCTATCAGTGATAGAGA
tet operator	tetO	—	1617..1636	Fwd
DNA Sequence TCCCTATCAGTGATAGAGA
tet operator	tetO	—	1653..1672	Fwd
DNA Sequence TCCCTATCAGTGATAGAGA
attB1	mutant version of attB	—	1825..1850	Fwd
DNA Sequence ACAAGTTTGTACAAAAAAGCAGGCT
attB2	mutant version of attB	—	3128..3153	Rev
DNA Sequence ACCACTTTGTACAAGAAAGCTGGGT
lox2272	—	—	3794..3828	Rev
DNA Sequence ATAACTTCGTATAAAGTATCCTATACGAAGTTAT
lac operator	—	—	4229..4246	Fwd
DNA Sequence TTGTTATCCGCTCACAA
CAP binding site	—	—	4298..4320	Fwd
DNA Sequence ATGAGTGAGCTAACTCACATTA

rep_origin 1

Label	Gene	Product	Position	Strand
ori	—	—	4607..5196	Rev
DNA Sequence TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA