pOsTIR1w/oGFP

102883 Oryza sativa Yeast Expression

Plasmid Information

Purpose
Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.
Depositor
Matthias Heinemann
Article
Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
Insert
OsTIR1
Tags
3x myc
Promoter
ADH1
Available Since
Jan. 5, 2018
Availability
Available

Sequence Information

Length
8,902 bp
Topology
circular
Molecule Type
other DNA

Features

CDS 6
Label Gene Product Position Strand
OsTIR1 rice transport inhibitor response 1 pro… 415..2140 Fwd
DNA Sequence
ATGACGTACTTCCCGGAGGAGGTGGTGGAGCACATCTTCAGCTTCCTGCCGGCGCAGCGCGACCGCAACACGGTCTCGCTCGTCTGCAAGGTGTGGTACGAGATCGAGAGGCTGAGCCGCCGCGGCGTCTTCGTGGGCAACTGCTACGCCGTGCGCGCCGGCCGCGTCGCCGCGCGGTTCCCCAACGTGCGGGCGCTCACGGTGAAGGGGAAGCCCCACTTCGCCGACTTCAACCTCGTGCCCCCCGACTGGGGCGGCTACGCGGGGCCGTGGATCGAGGCGGCCGCGAGGGGATGCCACGGCCTGGAGGAGCTCAGGATGAAGCGGATGGTGGTGTCCGACGAGAGCCTCGAGCTGCTGGCTCGCTCGTTCCCGCGGTTCAGGGCTCTTGTTCTTATCAGCTGCGAGGGGTTCAGCACTGACGGGCTAGCCGCCGTCGCGAGCCATTGCAAGCTTCTGAGGGAGTTGGATTTGCAGGAAAATGAAGTGGAGGATCGAGGGCCTAGGTGGCTTTCCTGCTTCCCTGATTCCTGCACATCACTTGTCTCATTGAATTTTGCCTGCATCAAAGGGGAGGTTAATGCTGGTTCACTGGAGAGACTTGTTAGCAGGTCCCCAAACCTGCGGAGTTTGAGGCTGAATCGATCTGTATCGGTAGATACACTTGCAAAGATACTACTGCGTACCCCTAACTTGGAGGATTTGGGGACAGGGAATTTGACAGATGACTTCCAAACTGAGTCCTACTTTAAGCTTACCAGTGCTCTGGAGAAATGCAAGATGTTGAGGAGTTTGTCTGGATTCTGGGATGCTTCTCCTGTTTGCCTGTCATTTATCTACCCCCTGTGTGCTCAACTGACAGGATTGAACTTGAGCTATGCACCCACACTTGATGCTTCTGACCTTACAAAAATGATTAGCCGCTGTGTGAAGCTCCAACGCCTTTGGGTACTGGATTGTATCTCGGACAAAGGCTTGCAAGTGGTGGCCTCCAGTTGCAAAGACTTGCAAGAACTCAGGGTATTTCCATCAGATTTCTACGTAGCTGGTTATTCTGCAGTGACAGAGGAGGGACTTGTTGCAGTATCCTTGGGCTGTCCAAAACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCACTAGTTACTGTCGCCAAGAACTGTCCAAATTTCACACGATTCAGACTTTGTATTCTTGAGCCAGGGAAGCCTGATGTTGTGACAAGCCAACCATTAGATGAAGGCTTTGGAGCTATTGTTCGTGAGTGCAAGGGATTACAACGTTTGTCAATATCTGGTCTTCTCACAGACAAAGTTTTCATGTATATTGGGAAATATGCAAAACAACTTGAGATGCTTTCTATAGCATTTGCTGGTGACAGTGATAAGGGTATGATGCATGTTATGAATGGATGCAAGAATTTAAGGAAACTGGAGATAAGAGATAGCCCGTTTGGTGATGCTGCACTCTTGGGGAATTTTGCTAGGTACGAGACAATGCGATCCCTTTGGATGTCATCTTGCAATGTCACGTTAAAGGGGTGCCAAGTCCTTGCGTCAAAGATGCCGATGCTCAATGTTGAGGTCATAAATGAGCGGGATGGTAGCAATGAAATGGAGGAAAACCATGGAGATCTGCCTAAAGTGGAGAAATTATATGTGTACCGCACAACTGCTGGGGCGAGGGATGATGCACCAAATTTTGTTAAAATCCTA
Protein Sequence
MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVGNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEELRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVEDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLAKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPLCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRVFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLCILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIAFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGCQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL
Myc Myc (human c-Myc proto-oncogene) epitop… 2167..2197 Fwd
DNA Sequence
GAACAAAAGTTGATTTCTGAAGAAGATTTG
Protein Sequence
EQKLISEEDL
Myc Myc (human c-Myc proto-oncogene) epitop… 2203..2233 Fwd
DNA Sequence
GAACAAAAGCTAATCTCCGAGGAAGACTTG
Protein Sequence
EQKLISEEDL
Myc Myc (human c-Myc proto-oncogene) epitop… 2239..2269 Fwd
DNA Sequence
GAACAAAAATTAATCTCAGAAGAAGACTTG
Protein Sequence
EQKLISEEDL
KanR aph(3')-Ia aminoglycoside phosphotransferase 2975..3785 Fwd
DNA Sequence
ATGGGTAAGGAAAAGACTCACGTTTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGCAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA
Protein Sequence
MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF
AmpR bla beta-lactamase 5614..6475 Fwd
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
promoter 4
Label Gene Product Position Strand
ADH1 promoter S. cerevisiae ADH1 0..8902 Fwd
DNA Sequence
GCATGCAACTTCTTTTCTTTTTTTTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAAAAAATGATGGAAGACACTAAAGGAAAAAATTAACGACAAAGACAGCACCAACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAAACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTATACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAAGTTTGCTGTCTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCTCGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATATTTCAAGCTATACCAAGCATACAATCAACT
TEF promoter 2631..2975 Fwd
DNA Sequence
GACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACC
T7 promoter 4596..4615 Rev
DNA Sequence
TAATACGACTCACTATAGG
AmpR promoter bla 5509..5614 Fwd
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT
protein_bind 2
Label Gene Product Position Strand
CAP binding site 7521..7543 Fwd
DNA Sequence
TAATGTGAGTTAGCTCACTCAT
lac operator 7597..7614 Fwd
DNA Sequence
TTGTGAGCGGATAACAA
rep_origin 1
Label Gene Product Position Strand
ori 6645..7234 Fwd
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
terminator 2
Label Gene Product Position Strand
ADH1 terminator S. cerevisiae ADH1 2418..2584 Fwd
DNA Sequence
GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTCTTATTCTTGAGTAACTCTTTCCTGTAGGTCAGGTTGCTTTCTCAGGTATAGCATGAGGTCGCTC
TEF terminator 3790..3988 Fwd
DNA Sequence
ACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG