| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| rop | rop | Rop protein, which maintains plasmids a… | 384..576 | Rev | |
|
DNA Sequence
GTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGA
Protein Sequence
MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL
|
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| 6xHis | — | 6xHis affinity tag | 2285..2303 | Fwd | |
|
DNA Sequence
CACCATCACCATCACCAT
Protein Sequence
HHHHHH
|
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| MBP | malE (mutated) | maltose binding protein from E. coli | 2312..3413 | Fwd | |
|
DNA Sequence
ATGAAAATCGAAGAAGGTAAACTGGTAATCTGGATTAACGGCGATAAAGGCTATAACGGTCTCGCTGAAGTCGGTAAGAAATTCGAGAAAGATACCGGAATTAAAGTCACCGTTGAGCATCCGGATAAACTGGAAGAGAAATTCCCACAGGTTGCGGCAACTGGCGATGGCCCTGACATTATCTTCTGGGCACACGACCGCTTTGGTGGCTACGCTCAATCTGGCCTGTTGGCTGAAATCACCCCGGACAAAGCGTTCCAGGACAAGCTGTATCCGTTTACCTGGGATGCCGTACGTTACAACGGCAAGCTGATTGCTTACCCGATCGCTGTTGAAGCGTTATCGCTGATTTATAACAAAGATCTGCTGCCGAACCCGCCAAAAACCTGGGAAGAGATCCCGGCGCTGGATAAAGAACTGAAAGCGAAAGGTAAGAGCGCGCTGATGTTCAACCTGCAAGAACCGTACTTCACCTGGCCGCTGATTGCTGCTGACGGGGGTTATGCGTTCAAGTATGAAAACGGCAAGTACGACATTAAAGACGTGGGCGTGGATAACGCTGGCGCGAAAGCGGGTCTGACCTTCCTGGTTGACCTGATTAAAAACAAACACATGAATGCAGACACCGATTACTCCATCGCAGAAGCTGCCTTTAATAAAGGCGAAACAGCGATGACCATCAACGGCCCGTGGGCATGGTCCAACATCGACACCAGCAAAGTGAATTATGGTGTAACGGTACTGCCGACCTTCAAGGGTCAACCATCCAAACCGTTCGTTGGCGTGCTGAGCGCAGGTATTAACGCCGCCAGTCCGAACAAAGAGCTGGCAAAAGAGTTCCTCGAAAACTATCTGCTGACTGATGAAGGTCTGGAAGCGGTTAATAAAGACAAACCGCTGGGTGCCGTAGCGCTGAAGTCTTACGAGGAAGAGTTGGCGAAAGATCCACGTATTGCCGCCACTATGGAAAACGCCCAGAAAGGTGAAATCATGCCGAACATCCCGCAGATGTCCGCTTTCTGGTATGCCGTGCGTACTGCGGTGATCAACGCCGCCAGCGGTCGTCAGACTGTCGATGAAGCCCTGAAAGACGCGCAGACT
Protein Sequence
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
|
|||||
| TEV site | — | tobacco etch virus (TEV) protease recog… | 3467..3488 | Fwd | |
|
DNA Sequence
GAAAACCTGTACTTCCAATCC
Protein Sequence
ENLYFQS
|
|||||
| AsCpf1 | — | CRISPR-associated protein from Acidamin… | 3497..7418 | Fwd | |
|
DNA Sequence
ATGACCCAGTTCGAGGGGTTTACCAATCTGTATCAAGTGAGCAAGACGCTGCGCTTTGAACTGATCCCACAGGGAAAAACCTTAAAACATATTCAAGAGCAGGGCTTTATCGAAGAAGATAAGGCCCGTAATGACCATTACAAAGAGTTAAAGCCGATTATTGATCGTATCTACAAGACCTATGCGGACCAGTGCTTACAATTGGTACAGCTTGATTGGGAGAACCTCTCTGCCGCCATCGATTCCTATCGTAAAGAAAAAACTGAAGAAACGCGCAACGCCCTGATTGAAGAGCAGGCCACCTATCGTAACGCGATTCATGACTATTTTATTGGCCGTACGGACAATCTGACGGACGCGATCAACAAGCGCCATGCGGAGATTTACAAAGGACTGTTTAAGGCTGAACTGTTCAATGGTAAGGTCCTTAAACAGCTTGGGACCGTCACAACGACGGAACATGAAAACGCGTTATTACGTAGCTTCGACAAGTTTACCACGTATTTCTCCGGCTTTTACGAAAATCGCAAAAACGTTTTCAGTGCCGAGGATATTTCCACTGCTATCCCTCATCGCATTGTGCAAGACAACTTCCCAAAATTCAAAGAAAATTGTCATATCTTCACCCGCTTAATCACCGCTGTACCGTCCCTGCGTGAGCATTTCGAAAACGTGAAAAAGGCCATTGGTATCTTCGTGTCTACTTCGATTGAGGAGGTATTTTCCTTTCCATTCTATAATCAGCTGCTGACCCAGACCCAAATTGATCTGTACAACCAGCTGCTTGGCGGTATTTCTCGTGAAGCAGGAACCGAAAAAATCAAAGGGTTGAACGAGGTGCTTAATCTGGCAATCCAGAAAAATGATGAAACCGCCCACATCATTGCTTCGTTACCTCATCGTTTTATCCCGTTGTTCAAGCAAATTTTAAGTGATCGCAATACGCTGTCGTTTATTCTGGAAGAATTCAAAAGTGATGAAGAGGTAATTCAGTCGTTTTGCAAATATAAAACCCTGTTACGTAACGAAAATGTCCTGGAAACAGCCGAGGCTTTGTTTAACGAACTGAATAGCATTGACCTGACGCATATCTTTATTAGCCACAAAAAATTAGAGACCATCTCATCAGCTCTGTGCGATCATTGGGATACACTGCGCAATGCGCTGTATGAACGTCGTATTTCGGAATTGACTGGCAAAATCACTAAAAGCGCGAAAGAGAAAGTACAGCGCTCGCTTAAACATGAAGATATCAACCTGCAGGAGATCATCAGCGCCGCGGGTAAAGAACTGTCGGAGGCATTTAAACAGAAGACGAGCGAGATTCTGTCCCACGCACATGCCGCCTTAGACCAGCCGCTCCCGACCACTCTGAAGAAACAGGAAGAGAAAGAAATCCTTAAAAGTCAACTGGACAGTTTACTGGGTCTCTATCATCTGCTGGATTGGTTTGCGGTAGACGAAAGCAATGAAGTGGATCCGGAGTTTAGTGCCCGTCTGACAGGAATCAAGCTGGAAATGGAGCCTTCGCTTAGCTTCTACAACAAAGCCCGCAATTATGCCACGAAAAAACCCTATAGTGTCGAAAAATTTAAACTCAACTTTCAAATGCCGACCCTTGCGTCGGGCTGGGATGTCAACAAAGAAAAAAACAACGGAGCTATTCTGTTCGTTAAAAATGGTCTGTACTACCTGGGCATCATGCCGAAACAGAAAGGTCGCTACAAAGCCCTTTCGTTCGAGCCCACGGAAAAAACAAGCGAAGGCTTCGACAAAATGTACTACGATTACTTTCCGGATGCAGCAAAAATGATCCCGAAATGTTCCACACAGCTGAAAGCCGTTACAGCACATTTTCAGACGCACACCACCCCCATCTTACTGTCCAACAATTTTATTGAACCGCTGGAGATTACTAAAGAAATTTATGATTTGAACAATCCGGAAAAAGAGCCAAAAAAGTTTCAAACCGCCTACGCTAAAAAAACCGGGGATCAGAAAGGGTACCGCGAAGCGTTGTGCAAGTGGATTGATTTCACCCGCGATTTTCTCAGTAAATATACCAAGACTACCTCGATTGACCTGAGCTCACTGCGCCCGAGCTCTCAATATAAGGATTTGGGTGAGTACTATGCTGAATTAAACCCTTTATTGTACCACATTTCTTTTCAGCGCATCGCCGAAAAGGAAATTATGGACGCAGTCGAAACCGGGAAACTGTACCTGTTCCAGATCTATAATAAGGACTTCGCCAAAGGACATCATGGCAAACCGAACCTGCACACCCTTTACTGGACCGGGCTTTTCTCTCCGGAAAATTTGGCGAAAACCTCGATCAAGCTTAACGGTCAAGCTGAGCTGTTTTACCGTCCAAAATCCCGCATGAAGCGCATGGCGCATCGTTTAGGTGAAAAAATGCTGAATAAGAAACTGAAAGATCAGAAAACCCCTATCCCGGATACCCTCTACCAGGAACTGTATGATTACGTGAACCATCGTCTCTCGCATGACCTGTCAGACGAAGCGCGTGCGTTACTGCCCAATGTAATCACAAAAGAAGTTTCGCATGAAATTATTAAAGATCGTCGTTTTACATCTGATAAATTCTTTTTTCATGTTCCGATCACCCTCAACTATCAGGCCGCAAACAGTCCAAGTAAGTTTAACCAGCGCGTTAATGCTTACCTGAAGGAACATCCGGAGACTCCGATTATTGGAATTGATCGCGGTGAACGTAATTTGATCTATATCACTGTGATCGATAGTACCGGTAAGATTCTGGAGCAGCGCAGCTTGAACACAATTCAACAGTTTGATTATCAGAAAAAATTAGACAACCGCGAAAAAGAGCGCGTGGCTGCCCGTCAGGCGTGGTCTGTTGTCGGTACCATTAAAGATCTGAAGCAGGGCTATCTTTCTCAGGTTATTCACGAAATTGTAGATCTGATGATCCATTATCAGGCGGTTGTTGTGTTGGAGAATCTCAATTTCGGTTTTAAGAGTAAGCGCACAGGCATCGCTGAAAAAGCAGTTTATCAGCAGTTTGAAAAAATGCTGATCGACAAATTGAACTGTTTAGTTCTCAAAGATTACCCAGCGGAAAAGGTGGGCGGAGTGCTGAATCCGTACCAATTAACGGATCAATTCACTTCCTTCGCAAAGATGGGTACCCAAAGCGGCTTTCTGTTCTATGTGCCGGCCCCGTATACCTCGAAAATCGATCCACTGACGGGCTTCGTAGATCCGTTCGTGTGGAAAACCATTAAAAATCATGAAAGTCGTAAACATTTTCTCGAAGGCTTCGACTTCCTGCACTACGACGTGAAAACTGGCGATTTCATTCTGCATTTTAAAATGAACCGCAACCTTTCGTTTCAGCGCGGTCTGCCGGGCTTTATGCCGGCTTGGGACATTGTTTTTGAGAAAAATGAAACCCAGTTTGATGCTAAAGGCACTCCTTTCATCGCCGGTAAACGCATCGTACCTGTGATTGAAAACCATCGTTTTACAGGGCGTTACCGTGATTTATACCCGGCGAACGAATTGATCGCGCTGCTGGAGGAAAAGGGCATCGTTTTCCGTGACGGCTCCAATATTCTGCCGAAATTACTGGAAAACGACGATTCACACGCAATTGATACCATGGTCGCACTGATTCGCTCAGTCTTACAGATGCGTAACTCTAATGCAGCCACAGGAGAAGATTATATTAATTCGCCAGTCCGCGATTTGAACGGTGTTTGCTTCGACAGCCGTTTTCAGAATCCTGAATGGCCGATGGACGCTGATGCCAACGGAGCTTATCATATCGCCCTGAAAGGCCAGCTCCTGCTGAACCACCTGAAGGAAAGCAAAGATCTGAAATTGCAGAACGGCATTAGCAACCAGGACTGGTTAGCATACATCCAGGAACTGCGTAAC
Protein Sequence
MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDHYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNAIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDKFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLNLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLRNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGKITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLKKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKARNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPLEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDLSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGHHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKDQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFHVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVVVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQFTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDVKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENHRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMRNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESKDLKLQNGISNQDWLAYIQELRN
|
|||||
| HA | — | HA (human influenza hemagglutinin) epit… | 7421..7448 | Fwd | |
|
DNA Sequence
TATCCCTATGACGTGCCCGATTATGCC
Protein Sequence
YPYDVPDYA
|
|||||
| HA | — | HA (human influenza hemagglutinin) epit… | 7427..7454 | Fwd | |
|
DNA Sequence
TATGACGTGCCCGATTATGCCAGCCTG
Protein Sequence
YDVPDYASL
|
|||||
| SV40 NLS | — | nuclear localization signal of SV40 (si… | 7466..7487 | Fwd | |
|
DNA Sequence
CCCAAGAAAAAACGCAAGGTG
Protein Sequence
PKKKRKV
|
|||||
| SV40 NLS | — | nuclear localization signal of SV40 (si… | 7493..7514 | Fwd | |
|
DNA Sequence
CCTAAGAAAAAGCGGAAAGTG
Protein Sequence
PKKKRKV
|
|||||
| mCherry | — | monomeric derivative of DsRed fluoresce… | 7553..8258 | Fwd | |
|
DNA Sequence
GTGAGCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTCCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGAGCTGTACAAG
Protein Sequence
VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
|
|||||
| SV40 NLS | — | nuclear localization signal of SV40 (si… | 8264..8285 | Fwd | |
|
DNA Sequence
CCTAAGAAAAAGCGAAAAGTT
Protein Sequence
PKKKRKV
|
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| AmpR | bla | beta-lactamase | 9089..9950 | Fwd | |
|
DNA Sequence
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
Protein Sequence
MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
|
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| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 promoter | — | — | 2136..2155 | Fwd | |
|
DNA Sequence
TAATACGACTCACTATAGG
|
|||||
| tet promoter | — | — | 8843..8872 | Rev | |
|
DNA Sequence
TTGACAGCTTATCATCGATAAGCTTTAAT
|
|||||
| AmpR promoter | bla | — | 8984..9089 | Fwd | |
|
DNA Sequence
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAACATTGAAAAAGGAAGAGT
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| ori | — | — | 10120..10709 | Fwd | |
|
DNA Sequence
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
|
|||||
| Label | Gene | Product | Position | Strand | |
|---|---|---|---|---|---|
| T7 terminator | — | — | 8430..8478 | Fwd | |
|
DNA Sequence
CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
|
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