mini-AID — PlasmidDB

Feature Information

Product

minimal degron from the auxin-responsive IAA17 protein of Arabidopsis thaliana (Kubota et al., 2013)

Note

acts as an auxin-inducible degron (AID) in the presence of TIR1

Type

CDS

Protein Sequence

68 amino acids

KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVKVSMDGAPYLRKIDLRMYK

Found in 4 Plasmids

Plasmid	Species	Position	Strand
RepairTemplate_Smc4-mAID-HaloTAG 217658	Homo sapiens	1004..1208	Rev
RepairTemplate_NIPBL_mEGFP_mAID 217664	Homo sapiens	1067..1271	Fwd
pFA6a-mAID-mNeon-HIS3MX6 247601	Arabidopsis thaliana	62..266	Fwd
pFA6a-mAID-mNeon-kITRP1 247602	Arabidopsis thaliana	62..266	Fwd

DNA Sequence Variants

Sequence context may differ across plasmids due to position and neighboring features.

RepairTemplate_Smc4-mAID-HaloTAG 217658

1004..1208 (Rev)

DNA Sequence (204 bp)

AAGGAGAAGAGTGCTTGTCCTAAAGATCCAGCCAAACCTCCGGCCAAGGCACAAGTTGTGGGATGGCCACCGGTGAGATCATACCGGAAGAACGTGATGGTTTCCTGCCAAAAATCAAGCGGTGGACCAGAGGCAGCTGCATTCGTGAAGGTATCAATGGACGGAGCACCATACTTGAGGAAAATCGATTTGAGGATGTATAAG

Protein Sequence (68 aa)

KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVKVSMDGAPYLRKIDLRMYK

RepairTemplate_NIPBL_mEGFP_mAID 217664

1067..1271 (Fwd)

DNA Sequence (204 bp)

Protein Sequence (68 aa)

KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVKVSMDGAPYLRKIDLRMYK

pFA6a-mAID-mNeon-HIS3MX6 247601

62..266 (Fwd)

DNA Sequence (204 bp)

AAGGAGAAGAGTGCTTGTCCTAAAGATCCAGCCAAACCTCCGGCCAAGGCACAAGTTGTGGGATGGCCACCGGTGAGATCATACCGGAAGAACGTGATGGTTTCCTGCCAAAAATCAAGCGGTGGCCCGGAGGCGGCGGCGTTCGTGAAGGTATCAATGGACGGAGCACCGTACTTGAGGAAAATCGATTTGAGGATGTATAAA

Protein Sequence (68 aa)

KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVKVSMDGAPYLRKIDLRMYK

pFA6a-mAID-mNeon-kITRP1 247602

62..266 (Fwd)

DNA Sequence (204 bp)

Protein Sequence (68 aa)

KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVKVSMDGAPYLRKIDLRMYK